Spectral imaging system
First Claim
1. An imaging spectroscopy system, comprising:
- a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefined spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector.
6 Assignments
0 Petitions
Accused Products
Abstract
An imaging system is disclosed comprising an illuminator which produces illumination of any desired pure wavelength or of any selected mixture of pure wavelengths simultaneously, which illuminates a sample without spatio-spectral artifacts using illumination optics designed for that purpose; imaging optics, which form an image of the sample at a detector or viewing port; and a detector. This enables imaging the complete spectral image cube for a sample by taking sequential images while illuminating with a series of pure wavelengths, with greater ease and economy than by means of tunable filters, interferometers and the like. It further enables imaging while the sample is illuminated with a precisely controlled mixture of illuminant wavelengths, so that the image presented to the detector is a linear superposition of the sample properties at many wavelengths. This enables taking images of a sample'"'"' that directly measure the weighted spectral properties such as projection pursuit vectors, principal components, and the like. Data acquisition is enormously simplified, and speed is increased by one to two orders of magnitude over existing techniques. This is of great benefit in pathology, immunohistochemistry, Pap smear analysis, endoscopy, counterfeit detection, quality control, and other areas where one wishes to measure a spectral index of a living or inert sample.
-
Citations
36 Claims
-
1. An imaging spectroscopy system, comprising:
-
a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefined spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector. - View Dependent Claims (2, 3, 4, 5)
-
-
6. A method of spectral imaging comprising:
-
obtaining reference images at a plurality of pure spectral bands by controlling a spectral illuminator to sequentially produce light in each of said plurality of pure spectral bands and recording an image under these conditions using an imaging detector;
calibrating the system spectral response using the reference images; and
measuring the value of one or more spectral weighting functions in the sample by repeating the following steps for each spectral weighting function;
i) adjusting the spectral illuminator to produce light whose spectral flux distribution is based upon the calibration data and the spectral weighting function;
ii) illuminating the sample with light from the spectral illuminator as adjusted in step (i); and
(iii) recording an image of the sample under these illumination conditions using the imaging detector, wherein each of said one or more spectral weighting functions indicates a property or attribute of the sample and comprises a plurality of components in different spectral bands. - View Dependent Claims (7, 8, 9, 10)
-
-
11. A method of imaging comprising:
-
obtaining images of a first sample at a plurality of pure spectral bands by controlling a spectral illuminator to sequentially produce light in each of said plurality of pure spectral bands and recording images of the first sample under these conditions using an imaging detector;
determining one or more spectral weighting functions that indicate a property or attribute of a sample, using the images of the first sample, wherein each spectral weighting function comprises a plurality of components in different spectral bands; and
measuring the value of one or more of said spectral weighting functions in a subsequent sample by repeating the following steps for each of said one or more spectral weighting functions;
i) adjusting the spectral illuminator to produce light whose spectral flux distribution is based upon the spectral weighting function, ii) illuminating the sample with light from the spectral illuminator as adjusted in step (i); and
iii) recording an image of the second sample under these illumination conditions using the imaging detector. - View Dependent Claims (12, 13, 14, 15)
-
-
16. A method of spectral imaging comprising:
-
obtaining reference images at a plurality of pure spectral bands by controlling a spectral illuminator to sequentially produce light in each of said plurality of pure spectral bands and recording an image under these conditions using an imaging detector;
calibrating the system spectral response using the reference images;
obtaining images of a first sample at a plurality of pure spectral bands by controlling a spectral illuminator to sequentially produce light in each of said plurality of pure spectral bands and recording images of the first sample under these conditions using an imaging detector;
determining one or more spectral weighting functions that indicate a property or attribute of a sample, using the images of the first sample, wherein each spectral weighting function comprises a plurality of components in different spectral bands; and
wherein a spectral weighting funks comprises a plurality of components in different spectral bands;
measuring the value of one or more spectral weighting functions in a subsequent sample by repeating the following steps for each of said one or more spectral weighting functions;
i) adjusting the spectral illuminator to produce light whose spectral flux distribution is based upon the calibration data and the spectral weighting function, and ii) illuminating the sample with light from the spectral illuminator as adjusted in step (i); and
iii) recording an image of the second sample under these illumination conditions using the imaging detector. - View Dependent Claims (17)
-
-
18. A pathology workstation comprising:
-
a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefie spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector. - View Dependent Claims (19, 20, 21, 22)
-
-
23. A blood analysis workstation comprising:
-
a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefined spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector. - View Dependent Claims (24, 25, 26, 27, 28)
-
-
29. An endoscopy imaging station comprising:
-
a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefined spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector. - View Dependent Claims (30, 31, 32)
-
-
33. A retinal imaging station comprising:
-
a spectral illuminator that emits light in a plurality of spectral bands within a predefined spectral range, with the intensity of light in each spectral band being independently adjustable;
drive circuitry that controls the flux in each spectral band of the spectral illuminator;
scrambling optics that spatially homogenize the distribution of light emitted by the spectral illuminator;
beam delivery optics that direct the spatially homogenized light from the scrambling optics to form an illumination pattern at a sample, wherein the illumination pattern is substantially free of variation as a function of wavelength within the predefined spectral range;
receiver optics that receive light which has interacted with the sample and form an image of the sample at a focal plane;
an imaging detector located at the focal plane for detecting an image of the sample; and
a computer in communication with the drive circuitry and the imaging detector for controlling the drive circuitry and receiving data from the imaging detector. - View Dependent Claims (34, 35)
-
-
36. A method of imaging comprising using a spectral illuminator, launch optics, imaging detector with step of designing launch optics to minimize spectro-spatial artifacts.
Specification