Methods and compositions for use of catalase in hydrogels and biosensors
First Claim
1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of catalase ranges from about 100 units/ml to about 1000 units/ml.
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Abstract
Hydrogels containing catalase co-immobilized with an analyte-sensitive enzyme such as glucose oxidase are disclosed. The hydrogels may be pH-sensitive, and preferably are thin and lightly crosslinked. The catalase is present in concentrations ranging generally from 100 units/ml to about 1000 units/ml. These hydrogels have much faster swelling response times as compared to hydrogels without catalase, and are useful in biosensors and analyte-responsive drug delivery devices. The hydrogels also have an increased useful life, due to protection of the immobilized analyte-sensitive enzyme from degradation by hydrogen peroxide.
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20 Claims
- 1. A polymer matrix incorporating catalase co-immobilized with an analytic enzyme which generates hydrogen peroxide, wherein the concentration of catalase ranges from about 100 units/ml to about 1000 units/ml.
- 7. A biosensor or analyte-responsive drug delivery device which contains a polymer matrix and an analytic enzyme that generates hydrogen peroxide, wherein the analytic enzyme is co-immobilized in the biosensor or drug delivery device with catalase at a concentration ranging from about 100 units/ml to about 900 units/ml.
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15. A method of making a polymer matrix for use in a biosensor or analyte-responsive drug delivery device containing an analytic enzyme that generates hydrogen peroxide, including a step of co-immobilizing the analytic enzyme with catalase at a concentration ranging from about 100 units/ml to about 1000 units/ml.
- 16. The method of claim 16, wherein the polymer matrix is formulated to have a crosslinking proportion of between about 0.5 mol % and about 6 mol %.
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19. A method of making a biosensor or analyte-responsive drug delivery device, comprising the steps of:
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providing an analyte-sensitive enzyme that generates hydrogen peroxide, providing catalase, providing a pre-gel solution of polymerizable constituents, combining the catalase at a concentration of between about 100 units/ml and about 1000 units/ml with the analytic enzyme in the pre-gel solution to produce an analytic pre-gel solution, placing the analytic pre-gel solution in an appropriate support structure, subjecting the analytic pre-gel solution to appropriate conditions to form a shaped polymer matrix having the catalase and the analytic enzyme co-immobilized therein, providing sensing components capable of detecting a change proportional to the activity of the analyte-sensitive enzyme, providing structural components necessary to construct a biosensor, and functionally associating the shaped polymer matrix with the sensing and structural components to produce a biosensor. - View Dependent Claims (20)
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Specification