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Use of azetidinone compounds

  • US 20020009714A1
  • Filed: 07/30/2001
  • Published: 01/24/2002
  • Est. Priority Date: 04/16/1999
  • Status: Active Grant
First Claim
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1. A method for identifying a protein involved in cholesterol absorption in a biological system comprising the steps of:

  • (a) providing a cDNA expression library capable of expressing a protein involved in cholesterol absorption in a biological system, said cDNA expression library comprising a plurality of cells capable of expressing different cDNAs;

    (b) screening said expression library by incubating cells from said library with a fluorescent cholesterol absorption inhibitor;

    (c) after step (b), identifying the cell or cells in said library that display the greatest amount of fluorescence; and

    (d) identifying the protein associated with the cDNA expressed by said cell or cells displaying the greatest amount of fluorescence.

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