Process for identification of genes encoding proteins having cell proliferation-promoting activity
First Claim
1. A process for identifying a cell proliferation gene comprising the steps of:
- (a) selecting a growth proficient revertant cell from a plurality of cultured cells arrested for growth, said growth arrested cells transformed with a library comprising a plurality of nucleic acid sequence inserts, wherein at least one insert from the library encodes a perturbagen within said revertant cell that results in said reversion to growth proficiency; and
(b) identifying one or more genes or gene products in said revertant cell that cause said reversion to growth proficiency.
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Abstract
The present invention is directed to selection systems for the identification of cell proliferation genes based on functional analysis. More specifically, the invention is directed to a process for the identification of a cell proliferation promoting activity, the isolation of genes involved in such cell proliferation promoting activity, and the use of the so identified genes for the diagnosis or treatment of a disease associated with excessive cell proliferation. The invention further is directed to the design and development of antibodies, peptides, nucleic acids, and other compounds which specifically interfere with the function of the identified gene and/or its gene product, and pharmaceutical compositions comprising such compounds, for the treatment of diseases associated with inappropriate or unregulated cell proliferation.
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Citations
46 Claims
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1. A process for identifying a cell proliferation gene comprising the steps of:
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(a) selecting a growth proficient revertant cell from a plurality of cultured cells arrested for growth, said growth arrested cells transformed with a library comprising a plurality of nucleic acid sequence inserts, wherein at least one insert from the library encodes a perturbagen within said revertant cell that results in said reversion to growth proficiency; and
(b) identifying one or more genes or gene products in said revertant cell that cause said reversion to growth proficiency. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
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Specification