Multi-well plate and method of manufacture
First Claim
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1. A multiwell plate for use in assaying samples, comprising:
- a frame that forms sidewalls of at least one well, the frame being formed from an organic polymeric material;
a layer that forms a bottom of the at least one well, the layer being formed from an inorganic material; and
. whereby the frame and the layer are covalently attached and bound without use of an adhesive.
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Abstract
A method of manufacture and assembly of multiwell plates employing targeted radiation at an interface in order to achieve bonding is disclosed. The method accommodates glass and polymer attachment as well as polymer to polymer attachment. Resultant plates have unique flatness and optical properties.
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Citations
50 Claims
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1. A multiwell plate for use in assaying samples, comprising:
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a frame that forms sidewalls of at least one well, the frame being formed from an organic polymeric material;
a layer that forms a bottom of the at least one well, the layer being formed from an inorganic material; and
. whereby the frame and the layer are covalently attached and bound without use of an adhesive. - View Dependent Claims (2, 3, 4, 5)
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6. A multiwell plate forming a plurality of sample wells for holding samples to be assayed, said plate comprising:
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a unitary upper plate forming the sidewalls of the sample wells, said upper plate formed from a polymeric material;
a unitary lower plate forming the bottom walls of the sample wells, said bottom wall having an upper surface and said lower plate comprised of an inorganic material; and
,whereby said upper plate is joined to said upper surface by means other than by adhesive attachment. - View Dependent Claims (7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19)
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13. A method of making a multiwell plate comprising the steps of:
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providing an upper plate having an array of open ended wells, said upper plate being formed from an organic polymeric material having a predetermined melting temperature;
providing a substantially flat transparent lower plate, said lower plate being comprised of an inorganic material;
contacting said upper plate to said lower plate at an interface;
heating said upper plate at said interface to the melting temperature of said polymeric material; and
whereby said upper plate and said lower plate are bonded together.
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20. A method of making a multiwell plate comprising the steps of:
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providing an upper plate having an array of open ended wells, said upper plate being formed from an organic polymeric material having a predetermined melting temperature;
providing a substantially flat transparent lower plate, said lower plate being comprised of an inorganic material;
heating said lower plate to the melting temperature of said polymeric material;
contacting said upper plate to said lower plate at an interface whereby said upper plate and said lower plate are bonded together. - View Dependent Claims (21, 22, 23, 24, 26, 27, 28, 29)
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25. A method of making a multiwell plate comprising the steps of:
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providing an upper plate having an array of open ended wells, said upper plate being formed from an organic polymeric material having a predetermined melting temperature, said upper plate further containing a plurality of metallic flecks integrally blended therethrough;
providing a substantially flat transparent lower plate, said lower plate being comprised of an inorganic material;
contacting said upper plate to said lower plate at an interface;
directing a beam of electromagnetic radiation to the upper plate at said interface through said lower plate and thereby heating said upper plate to the melting temperature of said polymeric material; and
,whereby said upper plate and said lower plate are bonded together.
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30. A method of constructing a multiwell plate comprising:
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providing an upper plate having an array of open ended wells;
providing a substantially flat lower plate;
contacting said upper plate to said lower plate at an interface;
providing an energy absorptive region at said interface;
heating said energy absorptive region using energy emitted from a radiation source; and
,whereby said radiation source does not physically contact said interface, said upper plate or said lower plate.
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31. A method of joining an organic polymeric material to glass comprising:
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providing a silane containing polymer part;
providing a glass part; and
,covalently bonding said polymer part to said glass part by means of siloxane linkages.
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32. A multiwell plate for use in assaying samples compromising in combination:
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an upper plate having at least one well therein extending between open ends on opposite surfaces of said upper plate;
a lower plate of polymer film having a thickness less than 5 mils and bonded to said upper plate by means other than adhesive attachment; and
whereby said lower plate extends across the at least one well creating a well bottom having a top surface. - View Dependent Claims (33, 34, 35, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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36. A multiwell plate for use in assaying samples comprising:
at least one well having sidewalls and a bottom, said bottom having a top surface having a reactive coating imparted thereupon and whereby said sidewalls are free of said coating.
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37. A method of making a multiwell plate comprising the steps of:
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providing an upper plate having an array of open ended wells, said upper plate being formed from an organic polymeric material having a predetermined melting temperature;
providing a lower plate, said lower plate being comprised of an organic polymeric material having a predetermined melting temperature;
contacting said upper plate to said lower plate at an interface, said interface capable of absorbing infra-red radiation; and
heating said interface to the melting temperature of either the polymeric material of the lower plate, the polymeric material of the upper plate, or both; and
whereby said upper plate and said lower plate are bonded together.
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50. A method of making a multiwell plate comprising the steps of:
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providing an upper plate having an array of open ended wells, said upper plate being formed from an organic polymeric material having a predetermined melting temperature;
providing a lower plate, said lower plate being comprised of an organic polymeric material having a predetermined melting temperature;
contacting said upper plate to said lower plate at an interface, said interface capable of absorbing electromagnetic radiation; and
heating said interface to the melting temperature of either the polymeric material of the lower plate, the polymeric material of the upper plate, or both; and
whereby said upper plate and said lower plate are bonded together.
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Specification