Micro-fluidic cell for optical detection of gases and method for producing same

• US 20020024662A1
• Filed: 08/21/2001
• Published: 02/28/2002
• Est. Priority Date: 08/22/2000
• Status: Active Grant

• Current Assignee: Nippon Telegraph and Telephone Corporation
• Original Assignee: Nippon Telegraph and Telephone Corporation
• Inventors: Morimoto, Takashi, Niwa, Osamu, Horiuchi, Tsutomu, Ueno, Yuko
• Granted Patent: US 6,600,558 B2
• Time in Patent Office: Days
• US Class Current: 356/246
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/058 Flat flow cell
• CPC Class Codes: G01N 2030/121 cooling; cold traps
• CPC Class Codes: G01N 2030/746 detecting along the line of flow, e.g. axial
• CPC Class Codes: G01N 2030/8411 Intermediate storage of effluent, including condensation on surface
• CPC Class Codes: G01N 21/0332 with temperature control control of temperature G05D23/00; cryostats F17C3/08
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3504 for analysing gases, e.g. multi-gas analysis
• CPC Class Codes: G01N 30/30 of temperature
• CPC Class Codes: G01N 30/74 Optical detectors measurement of intensity, velocity, spectral content, polarisation, or phase of infrared, visible or ultraviolet light G01J

Abstract

A micro-fluidic cell for optical detection of gases provided according to the present invention includes a concentration cell and a detection cell, and increases the sensitivity of optical detection of gases, selectivity of components, and accuracy of quantitative determination, and also achieves a low electric power consumption and a small-sized, light-weight configuration of the entire apparatus.

Claims

1. A micro-fluidic cell for optical detection of gases, comprising:

a microchannel through which gases to be analyzed flow;

a concentration cell; and

a detection cell, and wherein

said microchannel through which gases to be analyzed flow comprises a first microchannel including a gas inlet, a second microchannel including a gas outlet, and a connecting channel which connects said first microchannel and said second microchannel,

said concentration cell has said first microchannel, a substance adapted to adsorb and desorb the gases to be analyzed, and provided in part of said first microchannel, and a heating source for heating said substance for adsorbing and desorbing the gases to be analyzed, and

said detection cell has said second microchannel, an optical fiber for entry of ultraviolet light for spectrophotometric analysis into said second microchannel, and an optical fiber for exit of the ultraviolet light for spectrophotometric analysis from said second microchannel.

2. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein said concentration cell has a stopper for stopping said substance for adsorbing and desorbing the gases to be analyzed.

3. A micro-fluidic cell for optical detection of gases as claimed in claim 2, wherein said first microchannel has a microchannel for cold-trapping the gases to be analyzed which have been desorbed upon heating by said heating source.

4. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein said first microchannel and said second microchannel are formed on different substrates, the substrates are stacked, and said connecting channel is a through-hole.

5. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein said first microchannel, said second microchannel, and said connecting channel are formed on the same substrate.

6. A micro-fluidic cell for optical detection of gases as claimed in claim 3, wherein said first microchannel and said second microchannel are formed on different substrates, the substrates are stacked, and said connecting channel is a through-hole.

7. A micro-fluidic cell for optical detection of gases as claimed in claim 3, wherein said first microchannel, said second microchannel, and said connecting channels are formed on the same substrate.

8. A micro-fluidic cell for optical detection of gases as claimed in claim 3, wherein said microchannel for cold-trapping the gases to be analyzed is a channel free from a heater or a heat insulator and adapted to cool the gases by air cooling.

9. A micro-fluidic cell for optical detection of gases as claimed in claim 8, wherein a micro trench is provided in a top surface of the substrate corresponding to said microchannel for cold-trapping the gases to be analyzed.

10. A micro-fluidic cell for optical detection of gases as claimed in claim 9, wherein a cooling metallic thin film is provided in a bottom surface of the substrate corresponding to said microchannel for cold-trapping the gases to be analyzed.

11. A micro-fluidic cell for optical detection of gases as claimed in claim 8, wherein many micro pillars are provided in said microchannel for cold-trapping the gases to be analyzed.

12. A micro-fluidic cell for optical detection of gases as claimed in claim 11, wherein a cooling metallic thin film is provided in a bottom surface of the substrate corresponding to said microchannel for cold-trapping the gases to be analyzed.

13. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein a tip of said optical fiber for entry is semispherical, and a tip of said optical fiber for exit is flat.

14. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein a plurality of substances having different gas adsorbing and desorbing properties are used as said substance for adsorbing and desorbing the gases to be analyzed.

15. A micro-fluidic cell for optical detection of gases as claimed in claim 1, wherein said substance for adsorbing and desorbing the gases to be analyzed comprises at least one substance selected from the group consisting of porous glass, porous polymers, and porous carbon.

18. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 17, wherein the microchannel includes an adsorbent-loaded channel and a cold-trap channel, with the adsorbent stopper step being interposed therebetween.

19. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 18, further comprising a step of forming a micro trench on a surface of the second substrate opposite to a surface thereof facing the first substrate, and at a position corresponding to the cold-trap channel.

20. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 18, further comprising a step of forming a cooling metallic thin film on the bottom side of the first substrate and at a position corresponding to the cold-trap channel.

21. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 17, wherein said step of providing the heater is applied by a method selected from the group consisting of metal mask method, lift-off technique, plating, and ion milling.

22. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 17, wherein said step of forming the continuous trench pattern is applied by a method selected from the group consisting of a method using a dicing saw, wet etching, dry etching, and sand blasting.

24. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 23, wherein the microchannel of the first substrate includes an adsorbent-loaded channel and a cold-trap channel, with the adsorbent stopper step being interposed therebetween.

25. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 24, further comprising a step of forming a micro trench on the bottom side of the first substrate and at a position corresponding to the cold-trap channel.

26. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 24, further comprising a step of forming a cooling metallic thin film on the bottom side of the first substrate and at a position corresponding to the cold-trap channel.

27. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 23, wherein said step of providing the heater is applied by a method selected from the group consisting of metal mask method, lift-off technique, plating, and ion milling.

28. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 23, wherein said step of forming the continuous trench pattern is applied by a method selected from the group consisting of a method using a dicing saw, wet etching, dry etching, and sand blasting.

30. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 29, wherein the microchannel includes an adsorbent-loaded channel and a cold-trap channel, with the adsorbent stopper step being interposed therebetween.

31. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 30, further comprising a step of forming a micro trench on the bottom side of the first substrate and at a position corresponding to the cold-trap channel.

32. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 30, further comprising a step of forming a cooling metallic thin film on the bottom side of the first substrate and at a position corresponding to the cold-trap channel.

33. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 29, wherein said step of providing the heater is applied by a method selected from the group consisting of metal mask method, lift-off technique, plating, and ion milling.

34. A method for producing a micro-fluidic cell for optical detection of gases as claimed in claim 29, wherein said step of forming the united trench pattern is applied by a method selected from the group consisting of a method using a dicing saw, wet etching, dry etching, and sand blasting.

16. A gas trapping cell comprising:

a microchannel having a gas inlet and a gas outlet port and permitting flow of gases to be analyzed;

a substance provided in part of said microchannel and adapted to adsorb and desorb the gases to be analyzed;

a heating source for heating said substance for adsorbing and desorbing the gases to be analyzed; and

a microchannel for cold-trapping the gases to be analyzed which have been desorbed from said substance for adsorption and desorption provided in part of said microchannel.

17. A method for producing a micro-fluidic cell for optical detection of gases, comprising the steps of:

forming a trench in a continuous pattern including an adsorbent stopper step on a top side of a first substrate;

loading a substance for adsorbing and desorbing gases into part of the trench;

connecting an optical fiber for entry of ultraviolet light for spectrophotometric analysis and an optical fiber for exit of the ultraviolet light for spectrophotometric analysis with a predetermined spacing in the trench by use of an sealing material comprising glass;

bonding a second flat substrate to the top side of the first substrate by bonding to form a microchannel in the trench; and

providing a heater on a bottom side of the first substrate and at a position corresponding to a portion loaded with the substance for adsorbing and desorbing the gases.

23. A method for producing a micro-fluidic cell for optical detection of gases, comprising the steps of:

forming a trench in a continuous pattern including an adsorbent stopper step on a top side of a first substrate;

loading a substance for adsorbing and desorbing gases into part of the trench;

forming a trench in a predetermined continuous pattern on a top side of a second substrate and a through-hole in the trench;

connecting an optical fiber for entry of ultraviolet light for spectrophotometric analysis and an optical fiber for exit of the ultraviolet light for spectrophotometric analysis with a predetermined spacing in the trench of the second substrate by use of an sealing material comprising glass;

bonding a third flat substrate to the top side of the second substrate by bonding to form a microchannel in the trench of the second substrate;

bonding a bottom side of the second substrate to the top side of the first substrate by bonding to form a microchannel in the trench of the first substrate; and

providing a heater on a bottom side of the first substrate and at a position corresponding to a portion loaded with the substance for adsorbing and desorbing the gases.

29. A method for producing a micro-fluidic cell for optical detection of gases, comprising the steps of:

forming a trench in a continuous pattern including an adsorbent stopper step on a top side of a first substrate;

loading a substance for adsorbing and desorbing gases into part of the trench;

bonding a second flat substrate having a through-hole at a position corresponding to the trench of the first substrate to the top side of the first substrate by bonding to form a microchannel in the trench of the first substrate;

providing a heater on a bottom side of the first substrate and at a position corresponding to a portion loaded with the substance for adsorbing and desorbing the gases;

forming a trench in a predetermined continuous pattern on a top side of a third substrate and a through-hole in the trench;

connecting an optical fiber for entry of ultraviolet light for spectrophotometric analysis and an optical fiber for exit of the ultraviolet light for spectrophotometric analysis with a predetermined spacing in the trench of the third substrate by use of an sealing material comprising glass;

bonding a fourth flat substrate having a through-hole at a position corresponding to the trench of the third substrate to the top side of the third substrate by bonding to form a microchannel in the trench of the third substrate; and

stacking a fifth substrate comprising a Teflon seal packing interposed between the second substrate and the third substrate and having a through-hole at a position corresponding to the through-hole of the second substrate and the through-hole of the fourth substrate.

35. An apparatus for optical detection of gases, comprising:

a micro-fluidic cell for optical detection of gases, including a microchannel through which gases to be analyzed flow, a concentration cell, and a detection cell;

a heater power source;

an ultraviolet light source;

an ultraviolet spectrophotometer; and

a controller, and wherein

said microchannel through which the gases to be analyzed flow comprises a first microchannel including a gas inlet, a second microchannel including a gas outlet, and a connecting channel which connects said first microchannel and said second microchannel,

said concentration cell has said first microchannel, a substance adapted to adsorb and desorb the gases to be analyzed, and provided in part of said first microchannel, and a heating source for heating said substance for adsorbing and desorbing the gases to be analyzed, and said detection cell has said second microchannel, an optical fiber for entry of ultraviolet light for spectrophotometric analysis into said second microchannel, and an optical fiber for exit of the ultraviolet light for spectrophotometric analysis from said second microchannel.