Methods and apparatus for analyzing polynucleotide sequences

• US 20020025529A1
• Filed: 07/18/2001
• Published: 02/28/2002
• Est. Priority Date: 06/28/1999
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Unger, Marc, Quake, Stephen, Volkmuth, Wayne
• Granted Patent: US 6,911,345 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2300/14 Means for pressure control
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0655 pinch valves
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2533/101 Primer extension
• CPC Class Codes: C12Q 2535/125 Allele specific primer extension
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: F04B 43/043 Micropumps
• CPC Class Codes: F15C 5/00 Manufacture of fluid circuit elements; Manufacture of assemblages of such elements integrated circuits
• CPC Class Codes: F16K 2099/0074 using photolithography, e.g. etching
• CPC Class Codes: F16K 2099/0076 using electrical discharge machining [EDM], milling or drilling
• CPC Class Codes: F16K 2099/0078 : using moulding or stamping
• CPC Class Codes: F16K 2099/008 : Multi-layer fabrications
• CPC Class Codes: F16K 2099/0084 : Chemistry or biology, e.g. "lab-on-a-chip" technology
• CPC Class Codes: F16K 2099/0094 : Micropumps
• CPC Class Codes: F16K 99/0001 : Microvalves microdevices B81B1/00; manufacture or treatment of devices or systems in or on a substrate B81C1/00; microfluidic structures B01L3/5027; micropumps F04B19/006
• CPC Class Codes: F16K 99/0015 : Diaphragm or membrane valves
• CPC Class Codes: F16K 99/0051 : using electrostatic means
• CPC Class Codes: F16K 99/0059 : actuated by a pilot fluid
• CPC Class Codes: G01N 33/549 : with antigen or antibody entrapped within the carrier

Abstract

Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.

Claims

1. A method of analyzing a target polynucleotide comprising:

(a) providing a primed target polynucleotide attached to a microfabricated synthesis channel;

(b) flowing a first nucleotide through the synthesis channel under conditions whereby the first nucleotide attaches to the primer, if a complementary nucleotide is present to serve as template in the target polynucleotide;

(c) determining presence or absence of a signal, the presence of a signal indicating that the first nucleotide was incorporated into the primer, and hence the identity of the complementary base that served as a template in the target polynucleotide;

(d) removing or reducing the signal, if present; and

(e) repeating steps (b)-(d) with a further nucleotide, the same or different from the first nucleotide, whereby the further nucleotide attaches to the primer or a nucleotide previously incorporated into the primer.

2. The method of claim 1, wherein

step (a) comprises providing a plurality of different primed target polynucleotides attached to different synthesis channels;

step (b) comprises flowing the first nucleotide through each of the synthesis channels; and

step (c) comprises determining presence or absence of a signal in each of the channels, the presence of a signal in a synthesis channel indicating the first nucleotide was incorporated into the primer in the synthesis channel, and hence the identity of the complementary base that served as a template in the target polynucleotide in the synthesis channel.

3. The method of claim 2, wherein step (a) comprising providing a plurality of different primed target polynucleotides attached to each synthesis channel.

4. The method of claim 1, wherein said first nucleotide and said further nucleotide are labeled.

5. The method of claim 1, further comprising flushing the synthesis channel to remove unincorporated first or further labeled nucleotide.

6. The method of claim 4, wherein steps (b)-(d) are performed at least four times with four different types of labeled nucleotides.

7. The method of claim 4, wherein steps (b)-(d) are performed until the identity of each base in the target polynucleotide has been identified.

8. The method of claim 4, wherein said synthesis channel is formed by bonding a microfluidic chip to a flat substrate.

9. The method of claim 8, wherein said target polynucleotide is immobilized to the interior surface of said substrate in said synthesis channel.

10. The method of claim 9, wherein said interior surface is coated with a polyelectrolyte multilayer (PEM).

11. The method of claim 8, wherein said microfluidic chip is fabricated with an elastomeric materia.

12. The method of claim 11, wherein said an elastomeric material is RTV silicone.

13. The method of claim 4, wherein at least one of the labeled nucleotide comprises a mixture of labeled and unlabeled forms of the nucleotide.

14. The method of claim 4, wherein cross section of said synthesis channel has a linear dimension of less than 100 μ

m×

100 μ

m, less than 10 μ

m×

100 μ

m, less than 1 μ

m×

10 μ

m, or less than 0.1 μ

m×

10 μ

m.

15. The method of claim 4, wherein said label is a fluorescent label.

16. The method of claim 15, wherein said removing or reducing is by photobleaching.

17. The method of claim 4, wherein said label is a radiolabel.

18. The method of claim 17, wherein said removing or reducing is by chemical or enzymatic release of the label.

19. The method of claim 4, wherein said label is a mass-spectrometric label.

20. The method of claim 19, wherein said removing or reducing is by chemical or enzymatic release of the label.

21. The method of claim 1, wherein said signal is a non-optical signal.

22. The method of claim 21, wherein said non-optical signal is pyrophosphate release.

23. The method of claim 22, wherein said pyrophosphate release is detected with mass spectrometry.

24. The method of claim 22, wherein said pyrophosphate release is detected with an enzymatic reaction.

25. The method of claim 24, wherein said enzymatic reaction is a redox enzymatic reaction.

27. The method of claim 26, wherein said substrate is glass and said surface is coated with a polyelectrolyte multilayer (PEM).

28. The method of claim 27, wherein said PEM is terminated with a polyanion.

29. The method of claim 28, wherein said polyanion bears pendant carboxylic acid groups.

30. The method of claim 26, wherein said target polynucleotide is biotinylated, and said surface is coated with streptavidin.

31. The method of claim 30, wherein said surface is coated with biotin prior to coating with streptavidin.

32. The method of claim 31, wherein said surface is coated with a polyelectrolyte multilayer (PEM) terminated with carboxylic acid groups prior to attachment of biotin.

33. The method of claim 32, wherein said surface is pretreated with RCA solution prior to coating with said PEM.

35. The method of claim 34, wherein said label is a fluorescent label.

36. The method of claim 35, wherein said removing or reducing is by photobleaching.

37. The method of claim 36, wherein said fraction of the first nucleotide and said fraction of the further nucleotide are less than 10%.

38. The method of claim 37, wherein said fraction of the first nucleotide and said fraction of the further nucleotide are less than 1%.

39. The method of claim 38, wherein said fraction of the first nucleotide and said fraction of the further nucleotide are less than 0.1%.

40. The method of claim 34, wherein said fraction of the first nucleotide and said fraction of the further nucleotide are less than 0.01 %.

26. A method of analyzing a target polynucleotide comprising:

(a) pretreating the surface of a substrate to create surface chemistry that facilitates polynucleotide attachment and sequence analysis;

(b) providing a primed target polynucleotide attached to a surface of a substrate;

(c) providing a labeled first nucleotides to the attached target polynucleotide under conditions whereby the labeled first nucleotide attaches to the primer, if a complementary nucleotide is present to serve as template in the target polynucleotide;

(d) determining presence or absence of a signal, the presence of a signal indicating that the labeled first nucleotide was incorporated into the primer, and hence the identity of the complementary base that served as a template in the target polynucleotide; and

(e) repeating steps (c)-(d) with a labeled further nucleotide, the same or different from the first labeled nucleotide, whereby the labeled further nucleotide attaches to the primer or a nucleotide previously incorporated into the primer.

34. A method of analyzing a target polynucleotide comprising:

(a) providing a primed target polynucleotide;

(b) providing a first nucleotide under conditions whereby the first nucleotide attaches to the primer, if a complementary nucleotide is present to serve as template in the target polynucleotide;

wherein of said first nucleotide is labeled.

(c) determining presence or absence of a signal from the primer, the presence of a signal indicating the first nucleotide was incorporated into the primer, and hence the identity of the complementary base that served as a template in the target polynucleotide; and

(d) repeating steps (b)-(c) with a further nucleotide, the same or different from the first nucleotide, whereby the further nucleotide attaches to the primer or a nucleotide previously incorporated into the primer;

wherein a fraction of said further nucleotide is labeled.

41. An apparatus for analyzing the sequence of a polynucleotide, comprising:

(a) a flow cell comprising at least one microfabricated synthesis channel; and

(b) an inlet port and an outlet port, said inlet port and outlet port being in fluid communication with said flow cell for flowing fluids into and through said flow cell.

42. The apparatus of claim 41, furthering comprising a detector to detect a signal from said surface.

43. The apparatus of claim 42, furthering comprising a light source to illuminate the surface of said synthesis channel.

44. The apparatus of claim 42, wherein said signal is a fluorescent signal.

45. The apparatus of claim 42, wherein said signal is an electrochemical signal.

46. The apparatus of claim 41, wherein said synthesis channel is formed by bonding a microfluidic chip to a substrate.

47. The apparatus of claim 46, wherein said microfluidic chip further comprises microfabricated valves and microfabricated pumps in an integrated system with said microfabricated synthesis channel.

48. The apparatus of claim 47, further comprising a plurality of reservoirs for storing reaction reagents, wherein said microfabricated valve and said microfabricated pump are connected to said reservoirs.

49. The apparatus of claim 41,, wherein cross section of said synthesis channel has a linear dimension of less than 100 μ

m×

100 μ

m, less than 10 μ

m×

100 μ

m, less than 1 μ

m×

10 μ

m, or less than 0.1 μ

m×

1 μ

m.

50. The apparatus of claim 42, wherein said detector is a photon counting camera.

51. The apparatus of claim 46, wherein said microfluidic chip is fabricated with an elastomeric material.

52. The apparatus of claim 51, wherein said elastomeric material is RTV silicone.

53. The apparatus of claim 52, wherein said substrate is a glass cover slip.

54. The apparatus of claim 41, further comprising an appropriately programmed computer for recording identity of a nucleotide when said nucleotide becomes linked to a synthesis channel.