Method of measuring substance in sample using a redox reaction
First Claim
1. A method of measuring an analyte in a sample using a redox reaction, comprising:
- adding a tetrazolium compound to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance contained in the sample;
then forming a reducing substance or an oxidizing substance derived from the analyte;
measuring the quantity of the formed substance derived from the analyte by the redox reaction; and
determining the quantity of the analyte from the quantity of the formed substance derived from the analyte.
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Abstract
A highly reliable method of measuring an analyte in a sample using a redox reaction. In this method, a tetrazolium compound is added to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance in the sample, then a reducing substance or an oxidizing substance derived from the analyte is formed, the quantity of the formed substance derived from the analyte is measured by the redox reaction, and the quantity of the analyte is determined from the quantity of the formed substance derived from the analyte. As the tetrazolium compound, for example, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt can be used.
22 Citations
20 Claims
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1. A method of measuring an analyte in a sample using a redox reaction, comprising:
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adding a tetrazolium compound to a sample prior to the redox reaction so as to eliminate the influence of any reducing substance contained in the sample;
thenforming a reducing substance or an oxidizing substance derived from the analyte;
measuring the quantity of the formed substance derived from the analyte by the redox reaction; and
determining the quantity of the analyte from the quantity of the formed substance derived from the analyte. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification