Focused acoustic energy in the preparation of peptide arrays
First Claim
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1. A method for preparing an array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising applying focused acoustic energy to each of a plurality of reservoirs each containing a peptidic molecule in a fluid, wherein the focused acoustic energy is applied in a manner effective to eject a droplet from each reservoir toward a different site on a substrate surface.
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Abstract
The present invention relates to arrays of peptidic molecules and the preparation of peptide arrays using focused acoustic energy. The arrays are prepared by acoustically ejecting peptide-containing fluid droplets from individual reservoirs towards designated sites on a substrate for attachment thereto.
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Citations
60 Claims
- 1. A method for preparing an array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising applying focused acoustic energy to each of a plurality of reservoirs each containing a peptidic molecule in a fluid, wherein the focused acoustic energy is applied in a manner effective to eject a droplet from each reservoir toward a different site on a substrate surface.
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13. A method for preparing a peptide array comprised of a plurality of peptidic molecules attached to a substrate surface, the method comprising:
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(a) acoustically coupling a first reservoir containing a first peptidic molecule in a first fluid to an ejector that produces acoustic radiation;
(b) activating the ejector to generate acoustic radiation having a focal point sufficiently near the surface of the first fluid so as to eject a droplet thereof toward a first designated site on the substrate surface;
(c) acoustically coupling a second reservoir containing a second peptidic molecule in a second fluid to the ejector;
(d) activating the ejector as in step (b) to eject a droplet of the second fluid from the second reservoir toward a second designated site on the substrate surface; and
(e) repeating steps (c) and (d) with additional reservoirs each containing a peptidic molecule in a fluid until a droplet has been ejected from each reservoir.
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35. A method for preparing a peptide array comprised of a plurality of peptidic molecules attached to a porous substrate surface, the method comprising applying focused acoustic energy to each of a plurality of fluid-containing reservoirs each containing a peptidic molecule, wherein the focused acoustic energy is applied in a manner effective to eject a droplet having a volume of at most about 1 pL from each reservoir toward a different designated site on the porous substrate surface.
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38. A peptide array comprised of a plurality of peptidic molecules each attached through an optional linking moiety to a substrate surface, wherein substantially none of the peptidic molecules exhibits signs of shear stress and substantially all of the peptidic molecules are intact and attached to a predetermined site on the substrate surface.
- 55. A peptide array comprised of a plurality of peptidic molecules each attached through an optional linking moiety to a predetermined site on a porous substrate surface at a density of greater than 1,000,000 peptidic molecules per square centimeter of the substrate surface.
Specification