Device and method for lysing cells, spores, or microorganisms
First Claim
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1. A device for lysing components of a fluid sample, the device comprising:
- a) a cartridge having;
i) an inlet port for introducing the sample into the cartridge;
ii) a lysing chamber containing a membrane or filter for capturing the sample components as the sample flows through the lysing chamber;
iii) an outlet port for exit of the sample from the lysing chamber; and
iv) beads in the lysing chamber for rupturing the sample components; and
b) an ultrasonic transducer for transferring ultrasonic energy to the lysing chamber to agitate the beads and thereby rupture the components, wherein the transducer is coupled to a wall of the lysing chamber.
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Abstract
A device for lysing components (e.g., cells, spores, or microorganisms) of a fluid sample comprises a cartridge having a lysing chamber for receiving the sample and having at least one solid phase in the lysing chamber for capturing the sample components to be lysed. An ultrasonic transducer is coupled to a wall of the lysing chamber to transfer ultrasonic energy to the captured sample components.
262 Citations
21 Claims
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1. A device for lysing components of a fluid sample, the device comprising:
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a) a cartridge having;
i) an inlet port for introducing the sample into the cartridge;
ii) a lysing chamber containing a membrane or filter for capturing the sample components as the sample flows through the lysing chamber;
iii) an outlet port for exit of the sample from the lysing chamber; and
iv) beads in the lysing chamber for rupturing the sample components; and
b) an ultrasonic transducer for transferring ultrasonic energy to the lysing chamber to agitate the beads and thereby rupture the components, wherein the transducer is coupled to a wall of the lysing chamber. - View Dependent Claims (2, 3)
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4. A method for lysing components of a fluid sample, the method comprising the steps of:
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a) introducing the sample into a cartridge having;
i) a lysing chamber; and
ii) at least one solid phase positioned in the lysing chamber;
b) forcing the sample to flow through the lysing chamber to capture the sample components with the solid phase;
c) coupling an ultrasonic transducer to a wall of the lysing chamber; and
d) transferring ultrasonic energy from the transducer to the sample components. - View Dependent Claims (5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 19, 20, 21)
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12. A device for lysing components of a fluid sample, the device comprising:
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a) a cartridge having a lysing chamber for receiving the fluid sample;
b) at least one solid phase positioned in the lysing chamber for capturing the sample components to be lysed; and
c) an ultrasonic transducer for transferring ultrasonic energy to the captured sample components, wherein the cartridge includes at least one wall defining the lysing chamber, and wherein the transducer is coupled to the wall.
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18. A method for lysing components of a fluid sample, the method comprising the steps of:
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a) introducing the sample into a cartridge having a lysing chamber, wherein the lysing chamber contains a membrane or filter for capturing the sample components;
b) forcing the sample to flow through the lysing chamber to capture the sample components with the membrane or filter; and
c) agitating beads in the lysing chamber to lyse the sample components, wherein the beads are agitated by transferring ultrasonic energy to the lysing chamber using an ultrasonic transducer coupled to a wall of the chamber.
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Specification