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Method for separating analyte from a sample

  • US 20020042125A1
  • Filed: 11/07/2001
  • Published: 04/11/2002
  • Est. Priority Date: 08/13/1997
  • Status: Active Grant
First Claim
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1. A method for separating an analyte from a fluid sample and for concentrating the analyte into a volume of elution fluid, the method comprising the steps of:

  • a) introducing the sample into a cartridge having;

    i) a sample port;

    ii) a first flow path extending from the sample port;

    iii) an extraction chamber in the first flow path, wherein the extraction chamber contains at least one solid support for capturing the analyte from the sample; and

    iv) a second flow path for eluting the analyte from the extraction chamber, wherein the second flow path passes through the extraction chamber and diverges from the first flow path after passing through the extraction chamber;

    b) forcing the sample to flow through the first flow path, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber, wherein the ratio of the volume of sample forced to flow through the extraction chamber to the volume capacity of the extraction chamber is at least 2;

    1, and wherein the volume of sample forced to flow through the extraction chamber is at least 0.1 ml; and

    c) forcing an elution fluid to flow through the second flow path, thereby releasing the captured analyte from the solid support into the elution fluid.

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