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Bacterial two-hybrid system for protein-protein interaction screening, new strains for use therein, and their applications

  • US 20020045237A1
  • Filed: 03/28/2001
  • Published: 04/18/2002
  • Est. Priority Date: 03/29/2000
  • Status: Abandoned Application
First Claim
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1. A signal amplification system comprising a bacterial multi-hybrid system of at least two chimeric polypeptides containing:

  • (a) a first chimeric polypeptide corresponding to a first fragment of an enzyme;

    (b) a second chimeric polypeptide corresponding to a second fragment of an enzyme or a modulating substance capable of activating said enzyme, wherein the first fragment is fused to a molecule of interest and the second fragment or the modulating substance is fused to a target ligand and wherein the activity of the enzyme is restored by the in vivo interaction between the said molecule of interest and the said target ligand and wherein a signal amplification is generated; and

    wherein signal amplification is performed in an E. coli strain selected from the group consisting of strain BTH101 having C.N.C.M. Deposit Accession No. I-2309 and strain DHM1 having C.N.C.M. Deposit Accession No. I-2310.

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