Fluid cartridge and method

• US 20020045243A1
• Filed: 09/10/2001
• Published: 04/18/2002
• Est. Priority Date: 09/15/2000
• Status: Abandoned Application

• Current Assignee: Quantech Ltd.
• Original Assignee: Quantech Ltd.
• Inventors: Fischer, Vince, Laska, Ronald C., Thune, Greg, Uffenheimer, Kenneth, Brown, Jeffrey, Musgrave, Dwight
• Application Number:
  US09/952,115
• Publication Number:
  US 20020045243A1
• Time in Patent Office: Days
• US Class Current: 435/287.1
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/04 Exchange or ejection of cartridges, containers or reservoirs
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0672 Integrated piercing tool
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

Fluid flow cartridges, systems and methods for qualitative or quantitative analysis of a sample material are disclosed. Cartridges of the invention are constructed for fluid flow into, out of and internally throughout the cartridges. The cartridges can be used with an analytical instrument configured to interface with the instrument and present the sample or a reagent fluid at a location and in a form appropriate for analysis of the sample by the instrument. The invention is particularly advantageous for use in analytical systems in many fields but may also be used for non-analytical purposes.

Claims

1. A cartridge comprising:

a fluid reservoir for storage of a fluid;

a plurality of fluid passageways;

a selector valve for selectively controlling fluid flow through the fluid

passageways; and

an analyzer interface region for interfacing with a detector.

2. The cartridge according to claim 1 further comprising a sample receiving region for receiving a sample material.

3. The cartridge according to claim 1 further comprising a waste receptacle for receiving fluids passed through the cartridge.

4. The cartridge according to claim 2 wherein the sample receiving region is a reservoir integral to the cartridge.

5. The cartridge according to claim 3 wherein the waste receptacle is integral to the cartridge.

6. The cartridge according to claim 1 comprising a body including a valve chamber for receiving a valve core of the selector valve to direct fluid flow from a first fluid passageway of the plurality of fluid passageways to a second fluid passageway of the plurality of fluid flow passageways.

7. The cartridge according to claim 6 wherein the valve chamber includes a valve seat having a valve seat channel through which a fluid can flow from the first fluid passageway to the second fluid passageway.

8. The cartridge according to claim 7 wherein the valve seat channel provides for fluid flow from a driver inflow passageway to a reservoir inflow passageway.

9. The cartridge according to claim 7 wherein the valve seat channel provides for fluid flow from a reservoir outflow passageway to an analyzer passageway.

10. The cartridge according to claim 6 wherein the valve chamber includes a first valve seat channel through which a fluid can flow from the first fluid passageway to the second fluid passageway and a second valve seat channel through which a fluid can flow from a third fluid passageway to a fourth fluid passageway.

11. The cartridge according to claim 10 wherein the first fluid passageway is a driver inflow passageway, the second fluid passageway is a reagent inflow passageway, the third fluid passageway is a reagent outflow passageway and the fourth passageway is an analyzer passageway.

12. The cartridge according to claim 6 wherein the selector valve includes a fluid slot through which a fluid can flow from the first fluid passageway to the second fluid passageway.

13. The cartridge according to claim 12 wherein the valve seat channel can be in fluid communication with the fluid slot of the selector valve for fluid flow from the first fluid passageway to the second fluid passageway.

14. The cartridge according to claim 6 wherein the selector valve can be selectively positioned to direct fluid flow from the first fluid passageway to a third fluid passageway of the plurality of fluid flow passages.

15. The cartridge according to claim 1 including a conditioning region that can interface with a fluid conditioning element of an analytical instrument to condition a fluid flowing through the plurality of fluid passageways.

16. The cartridge according to claim 15 wherein the conditioning region provides for heating a fluid flowing through the plurality of fluid passageways.

17. The cartridge according to claim 16 wherein the fluid conditioned by the conditioning region is a sample material.

18. The cartridge according to claim 16 wherein the fluid conditioned by the conditioning region is a reagent fluid.

19. The cartridge according to claim 1 further comprising a filter region through which a fluid can be passed.

20. The cartridge according to claim 19 wherein the fluid passed through the filter is the sample material.

21. The cartridge according to claim 19 wherein the fluid passed through the filter is a reagent fluid.

22. The cartridge according to claim 2 wherein the sample receiving region can couple to a sample docking arrangement.

23. The cartridge according to claim 22 wherein the sample receiving region includes an arrangement for piercing a rubber stopper of a sample container in the sample docking arrangement.

24. The cartridge according to claim 1 wherein the cartridge includes a driver interface for receiving a driver to move fluid through the plurality of fluid passageways.

25. The cartridge according to claim 24 wherein the driver is air and the driver interface engages with a pump on an analytical instrument that forces air into the cartridge at the driver interface.

26. The cartridge according to claim 1 wherein the analyzer interface region includes a flow cell configured for interfacing with the detector of a surface plasmon resonance instrument.

27. The cartridge according to claim 3 wherein the waste receptacle includes a vent.

28. The cartridge according to claim 1 further comprising a body including:

a top surface and a bottom surface;

a plurality of surface channels, through body passageways and vias; and

a top membrane sealably attached to the top surface and a bottom

membrane sealably attached to the bottom surface.

29. The cartridge according to claim 27 wherein the body is injection molded.

30. The cartridge according to claim 1 wherein the detector is on an analytical instrument.

31. The cartridge according to claim 1 wherein the detector is a human.

32. A sample analysis system comprising:

an analytical instrument; and

a cartridge comprising;

a fluid reservoir for storage of a fluid;

a plurality of fluid passageways;

a selector valve for selectively controlling fluid flow through the fluid passageways;

an analyzer interface region for interfacing with a detector of the analytical instrument.

33. The sample analysis system according to claim 32 wherein the analytical instrument is a spectrophotometer.

34. The sample analysis system according to claim 32 wherein the analytical instrument is a surface plasmon resonance instrument.

35. The sample analysis system according to claim 32 wherein the cartridge comprises a body including a valve chamber for receiving a valve core of the selector valve to direct fluid flow from a first fluid passageway of the plurality of fluid passageways to a second fluid passageway of the plurality of fluid passageways.

36. The sample analysis system according to claim 35 wherein the analytical instrument includes a control center for controlling the direction of fluid flow through the selector valve.

37. The sample analysis system according to claim 32 wherein the cartridge includes a driver interface for receiving a driver to move fluid through the plurality of fluid passageways and the analytical instrument dispenses the driver to the driver interface.

38. The sample analysis system according to claim 34 wherein the driver is dispensed to the driver interface of the cartridge by a single stroke positive displacement pump.

40. The method according to claim 39 wherein the fluid is a sample material.

41. The method according to claim 40 wherein the sample material is introduced into the cartridge before the cartridge is placed in the analytical instrument.

42. The method according to claim 40 wherein the sample material is introduced into the cartridge by the analytical instrument.

43. The method according to claim 39 wherein the analytical instrument includes a driver dispenser to deliver the driver to the cartridge to drive the fluid through the cartridge.

44. The method according to claim 39 wherein the analytical instrument includes a control center for operating the selector valve.

45. The method according to claim 39 wherein the analytical instrument includes a control center for controlling the driver dispenser.

46. The method according to claim 39 wherein the control center is operated by a software program.

47. The method according to claim 43 wherein the fluid is a reagent fluid.

48. The method according to claim 39 wherein the selector valve includes a valve face comprising an elastomeric material having a first side and a second side wherein at least one of the first and second sides is covered by a flexible, low-friction, high tensile strength material.

39. A method for analyzing a sample material, the method comprising a step of:

placing a cartridge into an analytical instrument, the cartridge including;

(i) a plurality of fluid passageways

(ii) a selector valve to selectively direct flow of a fluid through the fluid passageways;

introducing a fluid into the cartridge;

driving the fluid through the cartridge with a driver;

operating the selector valve to selectively direct the flow of the fluid through the fluid passageways.