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Method for the detection of an analyte by means of a nucleic acid reporter

  • US 20020051986A1
  • Filed: 05/16/2001
  • Published: 05/02/2002
  • Est. Priority Date: 06/13/2000
  • Status: Active Grant
First Claim
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1. A method for the detection of an analyte comprising:

  • (i) contacting at least one analyte having at least two reporter conjugate binding sites with at least two reporter conjugates, said reporter conjugates each comprising;

    a) one member of a binding pair having specificity for at least one reporter conjugate binding site on said analyte;

    b) a nucleic acid label;

    wherein said analyte binds to said reporter conjugate forming an analyte dependent reporter complex;

    (ii) contacting said analyte dependent reporter complex with a enzyme composition wherein the nucleic acid labels on said reporter conjugates are joined to form an analyte specific amplicon;

    (iii) contacting the analyte dependent amplicon with an replication composition wherein amplification products are produced; and

    (iv) detecting said amplification products.

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