Three-dimensional probe carriers
First Claim
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1. A three dimensional probe array comprising a substrate formed of a substrate material and having multiple probe wells, each of said probe wells having a top surface, a bottom surface, an inner sidewall, an opening in said top surface, and an opening in said bottom surface;
- wherein a first probe well of said multiple probe wells contains probes, wherein the first probe well comprises a first light-conducting material and a second material, and wherein said first light-conducting material and said second material are configured such that a light beam launched into the opening of the first probe well in said top surface is transmitted by said first light-conducting material and exits the opening of said first probe well in said bottom surface.
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Abstract
The inventions disclosed herein provide a novel 3-dimensional arrangement of probes on a probe carrier with concomitant increases in the economy of reagents, compactness and readability of the probe carriers. Novel methods of fabricating probe carriers are also disclosed. Techniques for hybridization assays and reading results of such assays are also presented.
31 Citations
63 Claims
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1. A three dimensional probe array comprising a substrate formed of a substrate material and having multiple probe wells, each of said probe wells having a top surface, a bottom surface, an inner sidewall, an opening in said top surface, and an opening in said bottom surface;
- wherein a first probe well of said multiple probe wells contains probes, wherein the first probe well comprises a first light-conducting material and a second material, and wherein said first light-conducting material and said second material are configured such that a light beam launched into the opening of the first probe well in said top surface is transmitted by said first light-conducting material and exits the opening of said first probe well in said bottom surface.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 29, 30, 31, 32, 33, 34, 35, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 49, 50, 51, 52, 53, 54, 55, 56, 58, 59, 60)
- 28. An array of pillars comprising multiple pillars having proximal ends, distal ends, and sidewalls, wherein said proximal ends of said pillars are affixed to a surface of a substrate, and each of said pillars has a probe attached to the sidewalls of said pillars.
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36. A method of generating an array of capillaries comprising:
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a) forming an ordered bundle of a plurality of light conducting capillaries having distal and proximal ends and a channel extending from said distal end to said proximal end, wherein each said capillary is capable of conducting light parallel to the channel, and wherein the position of the distal end and proximal end of each said capillary are known;
b) securing the proximal ends of the capillaries to form a solid mass containing the proximal ends of said capillaries, said solid mass having a facet and said proximal ends being substantially coplanar at said facet; and
c) cutting said bundle into slices, wherein said capillaries are filled with biological or chemical samples prior to cutting into said slices.
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37. A method of generating an array of capillaries comprising:
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a) forming a random bundle of a plurality of capillaries having distal and proximal ends;
b) securing the proximal ends of the capillaries to form a solid mass containing the proximal ends of said capillaries, said solid mass having a facet and said proximal ends being substantially coplanar at said facet; and
c) cutting said bundle into slices, wherein said capillaries are filled with biological or chemical samples prior to cutting into slices.
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48. A method of manufacturing an array of pillars comprising:
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a) attaching biological or chemical samples to a sidewall of a plurality of pillars having proximal and distal ends;
b) coating said pillars with a removable protective layer;
c) forming a bundle of said pillars;
d) securing the proximal ends of said pillars to form a solid mass containing the proximal ends of the pillars, said solid mass having a facet and said proximal ends being substantially coplanar at said facet; and
e) cutting said bundle into slices.
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61. A method of detecting binding of a probe to target molecules adhered to an array of pillars comprising:
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a) providing an array of pillars comprising a plurality of pillars having proximal and distal ends, wherein said proximal ends are affixed to a substrate, and each of said pillars has a biological or chemical sample attached to the sidewalls of said pillar;
b) contacting the sidewalls of said pillars to a target molecule-containing fluid; and
c) detecting said target molecules bound to said chemical or biological samples. - View Dependent Claims (62, 63)
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Specification