Tissue ablation by shear force for sampling biological fluids and delivering active agents

• US 20020058902A1
• Filed: 04/30/2001
• Published: 05/16/2002
• Est. Priority Date: 05/01/2000
• Status: Active Grant

• Current Assignee: Cilag Gmbh International (Johnson & Johnson)
• Original Assignee: Lifescan Incorporated (Duv Holding Corporation)
• Inventors: Sun, Ying, Kollias, Nikiforos, Anthony F., Coston
• Granted Patent: US 7,404,815 B2
• Time in Patent Office: Days
• US Class Current: 604/20
• CPC Class Codes: A61N 1/303 Constructional details electrodes for external use A61N1/0428

Abstract

The present invention relates to a method and a device for transporting a molecule through a mammalian barrier membrane of at least one layer of cells comprising the steps of: ablating said membrane with a shear device; and utilizing a driving force to move said molecule through said perforated membrane.

Claims

1. A method for transporting a molecule through a mammalian barrier membrane of at least one layer of cells comprising the steps of:

ablating said membrane with a shear device comprising a sheet containing at least one opening and a shear member, where said sheet is contacted with said membrane such that a portion of said membrane is forced through said opening and ablates said portion of said membrane exposed through said opening; and

utilizing a driving force to move said molecule through said perforated membrane.

2. A method of claim 1, wherein said shear member is a shear blade.

3. A method of claim 2, wherein said portion of said membrane is forced into said opening by a pressure force.

4. A method of claim 3, wherein said pressure force is mechanical pressure.

5. A method of claim 3, wherein said pressure force is suction.

6. A method of claim 1, wherein said shear device further comprises a driving unit to move said blade.

7. A method of claim 6, wherein said driving unit is powered manually by the user of the device.

8. A method of claim 6, wherein said driving unit is powered by an electric motor.

9. A method of claim 1, wherein said membrane is selected from the group consisting of skin, buccal, vaginal, and rectal membranes.

10. A method of claim 1, wherein said membrane is human skin.

11. A method of claim 1, wherein said driving force is selected from a group consisting of iontophoresis, electro-osmosis, reverse iontophoresis, electroporation, phonophoresis, pressure gradients, and concentration gradients.

12. A method of claim 1, wherein said molecule is a pharmaceutical transported through said membrane into said mammal.

13. A method of claim 12, wherein said pharmaceutical is selected from the group consisting of polysaccharides, peptides, proteins, and polynucleotides.

14. A method of claim 12, wherein said molecule is a vaccine.

15. A method of claim 14, wherein said molecule is a vaccine against Staphylococcus aureus

16. A method of claim 1, wherein said molecule is transported from within said mammal out through said membrane.

17. A method of claim 16, wherein said molecule is glucose.

18. A method of claim 6, wherein said device further comprises a sensor, the feedback from said sensor modifies said driving unit.

19. A method of claim 18, wherein said sensor is selected from the group consisting of pressure sensor, conductivity sensor, impedance sensor, pH and temperature sensor.

20. A method of claim 1, wherein said sheer member moves parallel to said sheer sheet.

21. A method of claim 2, wherein said sheer blade moves parallel to said sheer sheet.

22. A method of claim 19, wherein said sensor is an impedance sensor measuring the impedance of the barrier membrane.

23. A method of claim 22, wherein the measurements from said impedance sensor are relayed to a microprocessor.