Method of stimulation hair growth

• US 20020065222A1
• Filed: 05/30/2001
• Published: 05/30/2002
• Est. Priority Date: 05/31/2000
• Status: Active Grant

• Current Assignee: Pioneer Surgical Technology Incorporated (Surgalign Holdings, Inc.)
• Original Assignee: Encelle, Inc. (Surgalign Holdings, Inc.)
• Inventors: Usala, Anton-Lewis
• Granted Patent: US 6,992,062 B2
• Time in Patent Office: Days
• US Class Current: 514/12
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 38/014 from connective tissue peptides, e.g. gelatin, collagen
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61K 8/042 Gels
• CPC Class Codes: A61K 8/65 Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/73 Polysaccharides
• CPC Class Codes: A61L 2430/18 for hair reconstruction
• CPC Class Codes: A61L 26/0052 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 26/008 Hydrogels or hydrocolloids
• CPC Class Codes: A61L 27/52 Hydrogels or hydrocolloids
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 17/14 for baldness or alopecia
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61Q 7/00 Preparations for affecting hair growth
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin

Abstract

The invention provides a method of stimulating hair growth, comprising administering a therapeutic amount of a hydrogel matrix to an intradermal or subdermal site where hair growth is desired, the matrix composition comprising gelatin, such as denatured collagen, and a long chain carbohydrate, such as dextran. The matrix may further include polar amino acids, nitric oxide inhibitors and super oxide inhibitors. Injection is a preferred method of administration.

Claims

1. A method of stimulating hair growth, comprising administering a therapeutic amount of a hydrogel matrix to an intradermal or subdermal site where hair growth is desired, the matrix composition comprising gelatin and a long chain carbohydrate.

2. The method of claim 1, wherein the matrix comprises about 0.01 to about 40 mM gelatin.

3. The method of claim 1, wherein the gelatin comprises denatured collagen.

4. The method of claim 1, wherein the long chain carbohydrate comprises dextran.

5. The method of claim 4, wherein the matrix comprises about 0.01 to about 10 mM dextran.

6. The method of claim 1, wherein the long chain carbohydrate has a molecular weight of about 20,000 to about 1,000,000 Daltons.

7. The method of claim 1, wherein the matrix further comprises an effective amount of polar amino acids selected from the group consisting of arginine, lysine, histidine, glutamic acid, and aspartic acid.

8. The method of claim 7, wherein the effective amount of polar amino acids comprises about 3 to about 150 mM of polar amino acids.

9. The method of claim 7, wherein the effective amount of polar amino acids comprises about 10 to about 65 mM of polar amino acids.

10. The method of claim 7, wherein the polar amino acids are selected from the group consisting of arginine, glutamic acid, lysine and mixtures thereof.

11. The method according to claim 10, wherein the matrix comprises:

about 2 to about 60 mM of L-glutamic acid;

about 0.5 to about 30 mM of L-lysine; and

about 1 to about 40 mM of arginine.

12. The method of claim 11, wherein the matrix comprises:

about 5 to about 40 mM of L-glutamic acid;

about 1 to about 15 mM of L-lysine; and

about 1 to about 30 mM of arginine.

13. The method according to claim 10, wherein the effective amount of polar amino acids comprises about 2 to about 60 mM of L-glutamic acid.

14. The method according to claim 10, wherein the effective amount of polar amino acids comprises about 1 to about 40 mM of arginine.

15. The method of claim 10, wherein the effective amount of polar amino acids comprises about 0.5 to about 30 mM of L-lysine.

16. The method of claim 1, wherein the matrix further comprises at least one nitric oxide inhibitor.

17. The method of claim 16, wherein the nitric oxide inhibitor is selected from the group consisting of L-cysteine, L-arginine analogues, cystine, heparin, and mixtures thereof.

18. The method of claim 16, wherein the nitric oxide inhibitor is present in an amount of about 5 to about 1000 μ

M.

19. The method of claim 16, wherein the nitric oxide inhibitor is present in an amount of about 20 to about 200 μ

M.

20. The method of claim 1, wherein the matrix further comprises about 5 to about 500 μ

M of L-cysteine.

21. The method of claim 20, wherein the matrix comprises about 15 to about 25 μ

M of L-cysteine.

22. The method of claim 1, wherein the matrix further comprises about 5 to about 500 μ

M of an L-arginine analogue.

23. The method of claim 22, wherein the L-arginine analogue comprises aminoguanidine.

24. The method of claim 22, wherein the matrix comprises about 15 to about 25 μ

M of an L-arginine analogue.

25. The method of claim 1, wherein the matrix further comprises a superoxide inhibitor.

26. The method of claim 25, wherein the superoxide inhibitor comprises EDTA or a salt thereof.

27. The method of claim 25, wherein the superoxide inhibitor is present in an amount of about 1 to about 8 mM.

28. The method of claim 1, wherein the gelatin comprises denatured collagen and the long chain carbohydrate comprises dextran.

29. The method of claim 1, wherein said administering step comprises injecting the matrix into one or more intradermal or subdermal locations.

30. A method of stimulating hair growth, comprising administering a therapeutic amount of a hydrogel matrix to an intradermal or subdermal site where hair growth is desired, the matrix composition comprising denatured collagen, dextran, and an effective amount of polar amino acids selected from the group consisting of arginine, lysine, histidine, glutamic acid, and aspartic acid.

31. The method of claim 30, wherein the effective amount of polar amino acids comprises about 3 to about 150 mM of polar amino acids.

32. The method of claim 31, wherein the effective amount of polar amino acids comprises about 10 to about 65 mM of polar amino acids.

33. The method of claim 30, wherein the polar amino acids are selected from the group consisting of arginine, glutamic acid, lysine and mixtures thereof.

34. The method according to claim 33, wherein the matrix comprises:

about 2 to about 60 mM of L-glutamic acid;

about 0.5 to about 30 mM of L-lysine; and

about 1 to about 40 mM of arginine.

35. The method of claim 30, wherein the matrix further comprises at least one nitric oxide inhibitor.

36. The method of claim 35, wherein the nitric oxide inhibitor is selected from the group consisting of L-cysteine, L-arginine analogues, cystine, heparin, and mixtures thereof.

37. The method of claim 35, wherein the nitric oxide inhibitor is present in an amount of about 5 to about 1000 μ

M.

38. The method of claim 35, wherein the nitric oxide inhibitor is present in an amount of about 20 to about 200 μ

M.

39. The method of claim 30, wherein the matrix further comprises about 5 to about 500 μ

M of L-cysteine.

40. The method of claim 30, wherein the matrix further comprises about 5 to about 500 μ

M of an L-arginine analogue.

41. The method of claim 30, wherein the matrix further comprises a superoxide inhibitor.

42. The method of claim 41, wherein the superoxide inhibitor comprises EDTA or a salt thereof.

43. The method of claim 30, wherein said administering step comprises injecting the matrix into one or more intradermal or subdermal locations.

44. A method of stimulating hair growth, comprising administering a therapeutic amount of a hydrogel matrix to an intradermal or subdermal site where hair growth is desired, the matrix composition comprising denatured collagen, dextran, aminoguanidine, L-cysteine, and an effective amount of polar amino acids selected from the group consisting of arginine, lysine, histidine, glutamic acid, and aspartic acid.

45. The method of claim 44, wherein said administering step comprises injecting the matrix into one or more intradermal or subdermal locations.