Proteomic analysis by parallel mass spectrometry
First Claim
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1. A method for analysis of proteins in a biological system comprising:
- providing a biological system;
exposing the system to a stimulus;
sampling the biological system at multiple time intervals after exposing the system to the stimulus, treating the multiple samples by separation technique to provide multiple protein samples suitable for analysis by mass spectrometry, and analyzing the multiple samples to determine changes in protein abundance as a function of time after exposing the biological system to stimulus, said analyzing including providing a parallel array of mass spectrometry systems adapted for protein analysis, and directing mass spectral data from the mass spectrometry systems in said array to a common computing device, said mass spectral data being indicative of the identity and the abundance of protein in said multiple sample, and correlating said mass spectral data as a function of time.
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Abstract
Analysis of, e.g., a proteome, utilizing a parallel array of mass spectrometers.
66 Citations
28 Claims
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1. A method for analysis of proteins in a biological system comprising:
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providing a biological system;
exposing the system to a stimulus;
sampling the biological system at multiple time intervals after exposing the system to the stimulus, treating the multiple samples by separation technique to provide multiple protein samples suitable for analysis by mass spectrometry, and analyzing the multiple samples to determine changes in protein abundance as a function of time after exposing the biological system to stimulus, said analyzing including providing a parallel array of mass spectrometry systems adapted for protein analysis, and directing mass spectral data from the mass spectrometry systems in said array to a common computing device, said mass spectral data being indicative of the identity and the abundance of protein in said multiple sample, and correlating said mass spectral data as a function of time. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 20, 21)
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19. A system for mass spectrometric analysis comprising:
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a parallel sample separation apparatus adapted to separate multiple samples in parallel for analysis by mass spectrometry, and a parallel array of mass spectrometry systems adapted to receive the samples from the separation apparatus, and a common computing device communicating with the parallel array of mass spectrometry systems and the parallel separation apparatus, the common computing device being adapted to analyze mass spectral data from the parallel array of mass spectrometry systems as function of sample identity.
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22. A method for analysis of proteins in a biological system comprising:
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providing a biological system containing proteins;
exposing the biological system to a stimulus;
after exposing the biological system to the stimulus, sampling the biological system at multiple time intervals to obtain multiple samples;
treating the multiple samples by a separation technique to provide multiple protein samples suitable for analysis by mass spectrometry;
providing a parallel array of mass spectrometer systems capable of simultaneous analysis of as many protein samples as there are spectrometer systems in said array;
analyzing the multiple protein samples in said parallel array of mass spectrometry systems to generate mass spectral data indicative of the identity and the abundance of proteins in said multiple protein samples; and
in a common electronic computing device communicating with each of said mass spectrometry systems, correlating said mass spectral data as a function of time. - View Dependent Claims (23, 24, 25, 26, 27, 28)
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Specification