MICROINCUBATOR COMPRISING A CELL CULTURE APPARATUS AND A TRANSPARENT HEATER

• US 20020072113A1
• Filed: 12/07/2000
• Published: 06/13/2002
• Est. Priority Date: 12/07/2000
• Status: Active Grant

• Current Assignee: BioCrystal, Ltd.
• Original Assignee: BioCrystal, Ltd.
• Inventors: Lucas, Rick D., Barbera-Guillem, Emilio
• Granted Patent: US 6,555,365 B2
• Time in Patent Office: Days
• US Class Current: 435/305.1
• CPC Class Codes: B01L 7/00 Heating or cooling apparatus autoclaves B01J3/04; Heat insulating devices
• CPC Class Codes: C12M 23/10 Petri dish crystallising dishes B01L3/06
• CPC Class Codes: C12M 23/22 Transparent or translucent parts glassware for laboratory use B01L3/00
• CPC Class Codes: C12M 23/24 Gas permeable parts
• CPC Class Codes: C12M 41/10 Filtering the incident radiation
• CPC Class Codes: C12M 41/22 in contact with the bioreactor walls

Abstract

Provided for heating cultured cells is a controlled heater device comprising a cell culture apparatus, a transparent heater in thermal contact with the cell culture apparatus, and may further comprise one or more of a transparent filter provided to prevent a predetermined spectral range of light from passing through the transparent filter, and a power source for controlling the amount of heat generated by the transparent heater of the controlled heater device.

Claims

1. A controlled heater device for heating cultured cells, the controlled heater device comprising:

a cell culture apparatus comprising at least one gas permeable, liquid impermeable membrane; and

at least one transparent heater in thermal contact with the cell culture apparatus.

2. The controlled heater device according to claim 1, wherein the controlled heater device provides an optical path that enables imaging of cells cultured in the controlled heater device.

3. The controlled heater device according to claim 1, wherein the at least one transparent heater is a transparent heater that comprises a laminate comprised of a metallic conductive material held in position between two transparent polymer sheets.

4. The controlled heater device according to claim 3, wherein the transparent heater further comprises a lead attachment area in which insulated lead wires are secured in electrical contact with the conductive material.

5. The controlled heater device according to claim 1, wherein the transparent heater is secured to a transparent, gas permeable, liquid impermeable membrane of the cell culture apparatus in forming the controlled heater device.

6. The controlled heater device according to claim 1, wherein the transparent heater is secured, in a leak-proof sealing, to a frame of the cell culture apparatus in forming the controlled heater device.

7. The controlled heater device according to claim 1, wherein the cell culture apparatus comprises multiple culture chambers.

8. The controlled heater device according to claim 1, further comprising a power source, a transparent filter, and a combination thereof.

9. The controlled heater device according to claim 8, wherein the controlled heater device further comprises a transparent filter provided in an optical path with respect to cells cultured in the controlled heater device, wherein the transparent filter prevents a predetermined spectral range of light from passing through the transparent filter.

10. The controlled heater device according to claim 9, wherein the predetermined spectral range of light is in a spectral range of from about 550 nm to about 700 nm.

11. The controlled heater device according to claim 9, wherein the transparent filter is gas-permeable.

12. The controlled heater device according to claim 9, wherein the transparent filter is secured to a surface selected from the group consisting of the transparent filter, the transparent, gas-permeable membrane, a frame of the cell culture apparatus, and a combination thereof.

13. The controlled heater device according to claim 9, wherein the transparent, gas-permeable membrane comprises the transparent filter.

14. The controlled heater device according to claim 9, wherein the transparent heater comprises the transparent filter.

15. The controlled heater device according to claim 9, wherein the transparent heater comprises the transparent filter and the transparent, gas-permeable membrane.

16. The controlled heater device according to claim 9, wherein the controlled heater device further comprises a power source for controlling the amount of heat generated by the transparent heater of the controlled heater device.

17. The controlled heater device according to claim 9, wherein the power source further comprises a temperature sensor.

18. The controlled heater device according to claim 17, wherein the temperature sensor senses the temperature of the transparent heater of the controlled heater device.

19. The controlled heater device according to claim 17, wherein the temperature sensor senses the temperature of contents in a culture chamber of the controlled heater device.

20. The controlled heater device according to claim 1, further comprising grid lines on an optical surface of the controlled heater device.

21. A controlled heater device for heating cultured cells, the controlled heater device comprising:

a cell culture apparatus comprising at least one transparent, gas permeable, liquid impermeable membrane;

a transparent heater in thermal contact with the cell culture apparatus; and

a transparent filter provided to prevent a predetermined spectral range of light from passing through the transparent filter;

wherein the transparent membrane, transparent heater, and transparent filter comprise optical surfaces that are aligned with respect to each other so as to provide an optical path that enables imaging of cells culture in the controlled heater device.

22. The controlled heater device according to claim 21, wherein the controlled heater device further comprises a power source for controlling the amount of heat generated by the transparent heater of the controlled heater device.

23. The controlled heater device according to claim 22, wherein the power source further comprises a temperature sensor for sensing the temperature of an object selected from the group consisting of the transparent heater, and contents in a culture chamber of the controlled heater device.

24. The controlled heater device according to claim 21, wherein the controlled heater device further comprises grid lines on an optical surface of the controlled heater device.

26. The transparent heater according to claim 25, wherein the transparent heater further comprises a lead attachment area in which insulated lead wires are secured in electrical contact with conductive material of the transparent heater.

27. The transparent heater according to claim 25, wherein the transparent heater is gas permeable.

25. A transparent heater comprising a transparent filter and a transparent liquid impermeable membrane, wherein the transparent filter is adapted to prevent a predetermined light spectral range from passing therethrough, and wherein the membrane is treated on a surface to promote adhesion of anchorage-dependent cells that come in contact with the treated surface.