Methods of enhancing SPLP-mediated transfection using endosomal membrane destabilizers
First Claim
Patent Images
1. A nucleic acid-lipid particle composition for introducing a nucleic acid into a cell, said particle comprising:
- a cationic lipid, a conjugated lipid that inhibits aggregation of particles, a nucleic acid and an endosomal membrane destabilizer.
1 Assignment
0 Petitions
Accused Products
Abstract
The present invention provides novel and surprisingly effective methods for delivering nucleic acids to cells. These methods are based upon the discovery that the presence of endosomal membrane destabilizers (e.g., calcium) leads to a dramatic increase in the transfection efficiency of plasmids formulated as SPLP, or “stabilized plasmid-lipid particles.”
-
Citations
68 Claims
-
1. A nucleic acid-lipid particle composition for introducing a nucleic acid into a cell, said particle comprising:
- a cationic lipid, a conjugated lipid that inhibits aggregation of particles, a nucleic acid and an endosomal membrane destabilizer.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
-
33. A method of introducing a nucleic acid into a cell, said method comprising:
contacting said cell with a nucleic acid-lipid particle composition, said particle comprising a cationic lipid, a conjugated lipid that inhibits aggregation of particles, and a nucleic acid; and
an endosomal membrane destabilizer.- View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63)
-
64. A method for inducing HII phase structure in a lipid bilayer, said method comprising:
- contacting said lipid bilayer with an endosomal membrane destabilizer, thereby inducing HII phase structure in a lipid bilayer.
- View Dependent Claims (65, 66, 67)
-
68. Use of nucleic acid-lipid particle composition for introducing a nucleic acid into a cell, said particle comprising:
- a cationic lipid, a conjugated lipid that inhibits aggregation of particles, a nucleic acid and an endosomal membrane destabilizer.
Specification