Apparatus and methods for separating components of particulate suspension

• US 20020076354A1
• Filed: 11/30/2001
• Published: 06/20/2002
• Est. Priority Date: 12/01/2000
• Status: Abandoned Application

• Current Assignee: Nagaoka & Co., Ltd.
• Original Assignee: Nagaoka & Co., Ltd.
• Inventors: Cohen, David Samuel
• Application Number:
  US09/997,895
• Publication Number:
  US 20020076354A1
• Time in Patent Office: Days
• US Class Current: 422/72
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0806 Standardised forms, e.g. compact disc [CD] format
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

An optical bio-disc used in separating components of particulate suspension has separation, fluid metering, and fluid assay chambers. The separation chamber contains a particulate suspension having a fluid component and a particulate matter component. The fluid metering chamber communicates with the separation chamber by a first conduit that has an entry point at the separation chamber. The entry point is accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber. The fluid assay chamber communicates with the fluid metering chamber by a second conduit.

Claims

1. An optical bio-disc for use in separating components of particulate suspension, comprising:

a separation chamber for containing a particulate suspension having a fluid component and a particulate matter component;

a fluid metering chamber communicating with the separation chamber by a first conduit, the first conduit having an entry point at the separation chamber, the entry point being accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber; and

a fluid assay chamber communicating with the fluid metering chamber by a second conduit.

2. The optical bio-disc of claim 1, wherein the fluid metering chamber has an elongated shape and the axis of rotation is radially closer to opposite ends of the fluid metering chamber than the axis of rotation is to the middle of the fluid metering chamber.

3. The optical bio-disc of claim 1, wherein opposite boundaries of the separation chamber are more than 10 degrees and less than 90 degrees in the same direction from a vector of rotation-induced centrifugal force extending through the center of the separation chamber.

4. The optical bio-disc of claim 1, further comprising:

an overflow chamber communicating with the fluid metering chamber by a third conduit.

5. The optical bio-disc of claim 1, further comprising:

an overflow chamber communicating with the fluid assay chamber by a third conduit.

6. The optical bio-disc of claim 1, wherein the fluid metering chamber has a U shape.

7. The optical bio-disc of claim 1, wherein the optical bio-disc has encoded information including instructions for analysis of the fluid assay chamber.

8. The optical bio-disc of claim 1, wherein the optical bio-disc has encoded information including instructions for controlling rotation of the bio-disc.

9. The optical bio-disc of claim 1, further comprising:

an antechamber in communication with the separation chamber by a third conduit.

10. The optical bio-disc of claim 9, wherein the third conduit includes first and second legs carrying particulate suspension in substantially opposite directions.

44. The optical bio-disc of claim 1, wherein the antechamber includes freeze-dried material.

45. The optical bio-disc of claim 1, wherein the antechamber includes anticoagulant.

46. The optical bio-disc of claim 1, wherein the freeze-dried material includes anticoagulant.

47. The optical bio-disc of claim 1, wherein the fluid metering chamber is configured to retain a controlled amount of fluid after termination of fluid flow through the fluid metering chamber.

48. The optical bio-disc of claim 1, wherein the location of the entry point at the separation chamber corresponds to an expected composition of the particulate suspension.

49. The optical bio-disc of claim 1, wherein the location of the entry point at the separation chamber corresponds to an expected volume of particulate matter component in the separation chamber.

50. The optical bio-disc of claim 1, wherein the fluid assay chamber further comprises a target zone bearing a bioactive agent.

51. The optical bio-disc of claim 1, further comprising a reaction chamber communicating with the fluid metering chamber.

52. The optical bio-disc of claim 1, further comprising a reaction chamber communicating with the fluid assay chamber.

53. The optical bio-disc of claim 1, wherein the fluid assay chamber further comprises first and second target zones bearing respective sets of bioactive agents, the first target zone being disposed upstream of the second target zone.

54. The optical bio-disc of claim 1, wherein the fluid assay chamber includes freeze-dried material.

55. The optical bio-disc of claim 51, wherein the reaction chamber includes freeze-dried material.

56. The optical bio-disc of claim 1, wherein the fluid assay chamber includes a bioactive agent.

57. The optical bio-disc of claim 51, wherein the reaction chamber includes a bioactive agent.

11. An optical bio-disc, comprising:

a substrate having encoded information associated therewith, the encoded information being readable by a disc drive assembly to control rotation of the disc;

an antechamber associated with the substrate;

a separation tube associated with the substrate, the separation tube in fluid communication with the antechamber;

a metering chamber associated with the substrate, the metering chamber in fluid communication with the separation tube; and

an assay zone associated with the substrate, the assay zone in fluid communication with the metering chamber so that when a particulate suspension including a particulate matter component and a liquid component is deposited into the antechamber, rotating the substrate in a predetermined manner delivers a metered amount of the liquid component to the assay zone.

13. The optical bio-disc of claim 12 wherein the substrate includes encoded information readable by a disc drive assembly to control rotation of the disc.

14. The optical bio-disc of claim 11 wherein the disc drive assembly includes a read beam enabled to analyze the liquid component in the assay zone.

16. The fluidic circuit of claim 15 wherein the particulate suspension includes a blood sample, the particulate matter component includes at least one from the group of white blood cells and red blood cells, and the liquid component includes serum.

17. The fluidic circuit of claim 15 wherein the particulate suspension includes a urine sample, the particulate matter component includes at least one from the group of epithelial cells, casts, and bacteria, and the liquid component includes clarified urine.

18. The fluidic circuit of claim 15 wherein the particulate suspension includes an environmental water sample, the particulate matter component includes at least one from the group of dirt, biological matter, particulate contaminants, and bacteria, and the liquid component includes clarified water.

19. The fluidic circuit of claim 16 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the serum.

20. The fluidic circuit of claim 17 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the clarified urine.

21. The fluidic circuit of claim 18 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the clarified water.

22. The fluidic circuit of claim 15 wherein the particulate suspension includes a blood sample, the particulate matter component includes at least one from the group of white blood cells and red blood cells, and the liquid component includes serum.

23. The fluidic circuit of claim 15 wherein the particulate suspension includes a urine sample, the particulate matter component includes at least one from the group of epithelial cells, casts, and bacteria, and the liquid component includes clarified urine.

24. The fluidic circuit of claim 15 wherein the particulate suspension includes an environmental water sample, the particulate matter component includes at least one from the group of dirt, biological matter, and particulate contaminants, and the liquid component includes clarified water.

25. The fluidic circuit of claim 22 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the serum.

26. The fluidic circuit of claim 23 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the clarified urine.

27. The fluidic circuit of claim 24 wherein when the disc is processed in an optical drive, a read beam is directed at the assay zone to analyze the clarified water.

29. The drive assembly of claim 28 further including means for displaying results from analyzing the return beam.

30. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of amniotic fluid, the particulate matter component includes at least one from the group of sloughed cells, cell debris, cells, vernix, and bacteria, and the liquid component includes clarified amniotic fluid.

31. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of cerebrospinal fluid, the particulate matter component includes at least one from the group of cell debris, cells, clots, and bacteria, and the liquid component includes clarified cerebrospinal fluid.

32. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of synovial fluid, the particulate matter component includes at least one from the group of cell debris, cells, clots, and bacteria, and the liquid component includes clarified synovial fluid.

33. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of pleural fluid, the particulate matter component includes at least one from the group of cell debris, cells, lipid, and bacteria, and the liquid component includes clarified pleural fluid.

34. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of pericardial fluid, the particulate matter component includes at least one from the group of cell debris, cells, lipid, and bacteria, and the liquid component includes clarified pericardial fluid.

35. The optical bio-disc of claim 11 wherein the particulate suspension includes a sample of peritoneal fluid, the particulate matter component includes at least one from the group of cell debris, cells, lipid, and bacteria, and the liquid component includes clarified peritoneal fluid.

38. The disc of claim 37, wherein the metering chamber is U-shaped with a bight portion at the outermost radial point of the metering chamber.

39. The disc of claim 37, wherein the metering chamber is substantially symmetric about a radius from an axis of rotation of the disc.

12. An optical bio-disc, comprising:

a substrate having a center and an outer edge;

an antechamber associated with the substrate;

a separation tube associated with the substrate, the separation tube in fluid communication with the antechamber;

a metering chamber associated with the substrate, the metering chamber in fluid communication with the separation tube;

an assay zone associated with the substrate, the assay zone in fluid communication with the metering chamber; and

a waste chamber associated with the substrate, the waste chamber in fluid communication with the meeting chamber so that when a particulate suspension including a particulate matter component and a liquid component is deposited into the antechamber, rotating the substrate in a predetermined manner delivers a metered amount of the liquid component to the assay zone while an excess amount of the liquid component is delivered to the waste chamber.

15. A fluidic circuit in a substrate for separating and metering a liquid component of a particulate suspension from particulate matter associated therewith, the fluidic circuit comprising:

an antechamber;

a separation tube in fluid communication with the antechamber;

a metering chamber in fluid communication with the separation tube;

an assay zone in fluid communication with the metering chamber; and

a waste chamber in fluid communication with the meeting chamber so that when a particulate suspension including a particulate matter component and a liquid component is deposited into the antechamber, causing the particulate suspension flow through the separation tube and the metering chamber delivers a metered amount of the liquid component to the assay zone while an excess amount of the liquid component is delivered to the waste chamber.

28. An optical bio-disc drive assembly, comprising:

a motor for rotating a respective optical bio-disc;

means for controlling the motor so that the bio-disc is rotated in a predetermined manner;

a source of electromagnetic radiation;

means for focusing the electromagnetic radiation at a predetermined location on the optical bio-disc;

means for receiving a return beam returned from an assay zone associated with the bio-optical disc, the assay zone including a liquid component of a particulate suspension which was separated from particulate matter associated therewith by rotating the optical bio-disc in the predetermined manner; and

means for analyzing the return beam so that desired characteristics of the liquid component are determined.

36. A rotatable disc for use in separating components of particulate suspension, comprising:

an separation chamber, elongated along a line, for containing a particulate suspension having a fluid component and a particulate matter component, the separation chamber being oriented at an angle that is in a range of 0-45 degrees relative to a vector of rotation-induced centrifugal force extending through the center of the separation chamber; and

a fluid metering chamber communicating with the separation chamber by an entry point at the separation chamber, the entry point being accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber.

37. A rotatable disc for use in separating components of particulate suspension, comprising:

a metering chamber formed in a substrate of the disc for receiving a liquid during a first rotation phase, and for delivering the liquid to another chamber in the substrate in a second rotating phase.

40. A method for use in separating components of particulate suspension, comprising:

at a first sustained speed, rotating an apparatus including a fluidic circuit having a separation component for holding the particulate suspension, a metering component communicating with the separation component, and an assay component communicating with the metering component, wherein centrifugal force resulting from the first sustained speed is insufficient to cause fluid of the particulate suspension to move from the separation component to the metering component;

rotating the apparatus at a second sustained speed causing centrifugal force that is sufficient to cause the fluid to move from the separation component to the metering component and being insufficient to cause the fluid to move from the metering component to the assay component; and

rotating the apparatus at a third sustained speed causing centrifugal force that is sufficient to cause the fluid to move from the metering component to the assay component.

41. An optical disc for separating components of particulate suspension, comprising:

a main chamber having a separation chamber for containing a particulate suspension having a fluid component and a particulate matter component and a fluid metering chamber communicating with the separation chamber by a first conduit, the first conduit having an entry point at the separation chamber, the entry point being accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber;

a reaction chamber in communication with the main chamber; and

a capture area in communication with the reaction chamber.

42. An optical disc for separating components of particulate suspension, comprising:

a main chamber;

a reaction chamber in communication with the main chamber, the reaction chamber having a separation chamber for containing a particulate suspension having a fluid component and a particulate matter component and a fluid metering chamber communicating with the separation chamber by a first conduit, the first conduit having an entry point at the separation chamber, the entry point being accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber; and

a capture area in communication with the reaction chamber.

43. A rotatable disc for use in separating components of particulate suspension, comprising:

an separation chamber, elongated along a line, for containing a particulate suspension having a fluid component and a particulate matter component, the separation chamber being oriented at an angle of approximately 30 degrees relative to a vector of rotation-induced centrifugal force extending through the center of the separation chamber; and

a fluid metering chamber communicating with the separation chamber by an entry point at the separation chamber, the entry point being accessible to the fluid component when the bio-disc is rotated causing separation of the fluid component and the particulate matter component in the separation chamber.