Fluorescence and FRET based assays for biomolecules on beads
First Claim
1. A sensing device comprising:
- a vessel;
a plurality of sensor beads located within said vessel to form interstitial spaces therethrough; and
a plurality of biomolecules bound to at least a portion of said plurality of beads, each of said biomolecules having a fluorescent tag.
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Abstract
The invention provides a sensing device comprising: a vessel; a plurality of sensor beads located within the vessel to form interstitial spaces therethrough; and a plurality of biomolecules bound to at least at portion of the plurality of beads, each of the biomolecules having a fluorescent tag. The invention also provides a method for detecting the binding of two biomolecules comprising the following steps: providing a plurality of first biomolecules, each of the first biomolecules having a first fluorescent tag, each of the first biomolecules being bound to a respective substrate of a plurality of substrate; providing a plurality of second biomolecules, each of the second biomolecules having a second fluorescent tag, binding at least portion of the second biomolecules to at least a portion of the first biomolecules to form complexes, wherein the plurality of first biomolecules and the plurality of second biomolecules prior to the binding step have a pre-complexing total fluorescence and wherein the complexes and free second biomolecules after the binding step have a post-complexing total fluorescence; and detecting any difference between the pre-complexing total fluorescence and the post-complexing total fluorescence. A sensing device comprising a suspension of a plurality of sensor beads; and a plurality of biomolecules bound to at least a portion of the plurality of beads, each of the biomolecules having a fluorescent tag is also provided.
84 Citations
51 Claims
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1. A sensing device comprising:
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a vessel;
a plurality of sensor beads located within said vessel to form interstitial spaces therethrough; and
a plurality of biomolecules bound to at least a portion of said plurality of beads, each of said biomolecules having a fluorescent tag. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 48, 49, 50, 51)
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28. A method for detecting the binding of two biomolecules comprising the following steps:
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providing a plurality of first biomolecules, each of said first biomolecules having a first fluorescent tag, each of said first biomolecules being bound to a respective substrate of a plurality of substrates;
providing a plurality of second biomolecules, each of said second biomolecules having a second fluorescent tag;
binding at least portion of said second biomolecules to at least a portion of said first biomolecules to form complexes, wherein said plurality of first biomolecules and said plurality of second biomolecules prior to said binding step have a pre-complexing total fluorescence and wherein said complexes and free second biomolecules after said binding step have a post-complexing total fluorescence; and
detecting any difference between said pre-complexing total fluorescence and said post-complexing total fluorescence.
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47. A sensing device comprising:
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a suspension of a plurality of sensor beads; and
a plurality of biomolecules bound to at least a portion of said plurality of beads, each of said biomolecules having a fluorescent tag.
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Specification