Analytical method and apparatus for liquid sample using near infrared spectroscopy

• US 20020084415A1
• Filed: 03/16/2001
• Published: 07/04/2002
• Est. Priority Date: 10/17/2000
• Status: Abandoned Application

• Current Assignee: Bio-Oriented Technology Research Advancement Institution, Japan As Represented By Director of National Food Research Institute Ministry of Agriculture Forestry and Fisheries
• Original Assignee: Bio-Oriented Technology Research Advancement Institution, Japan As Represented By Director of National Food Research Institute Ministry of Agriculture Forestry and Fisheries
• Inventors: Iyo, Chie, Kawano, Sumio
• Application Number:
  US09/810,278
• Publication Number:
  US 20020084415A1
• Time in Patent Office: Days
• US Class Current: 250/339.09
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/276 with alternation of sample and standard in optical path
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 2201/0227 Sealable enclosure
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 2201/129 Using chemometrical methods

Abstract

First, monochromatic near infrared light in a wavelength range of 700 nm-1100 nm from the slit of the near infrared apparatus 1 is applied to a reference ceramic plate through the optical fiber 7 to measure a transmitted light intensity of the ceramic plate which is a reference material for spectrum measurement. Next, in place of the ceramic plate, the test tube 4 containing a liquid sample of which the temperature has been adjusted at a predetermined temperature by a water bath and the like is inserted into the housing portion 5. The transmitted light intensity of the liquid sample is then measured using the same procedure as above. A so-called near infrared absorption spectrum in which absorbance has been plotted against wavelengths is displayed on the screen of the computer 2. Information about each object characteristic is extracted from the spectrum data using a calibration equation.

Claims

1. An analytical method for a liquid sample using near infrared spectroscopy comprising the steps of:

applying near infrared light in a short wavelength range to the liquid sample within a test tube from the outside;

detecting diffusely (scattered) reflected light, diffusely transmitted light, or transmitted and reflected light from the liquid sample by an optical sensor to measure a near infrared absorption spectrum of the liquid sample; and

replacing the measured value with a value obtained from a calibration equation which has been made in advance from a spectrum measured using the same method as above, thereby determining object characteristics of the liquid sample.

2. The analytical method for a liquid sample using near infrared spectroscopy according to claim 1, wherein the wavelength of near infrared light applied to the liquid sample is 700 nm˜

1100 nm.

3. The analytical method for a liquid sample using near infrared spectroscopy according to claim 1, wherein the liquid samples are food (such as milk, fruit and vegetable juice, and edible oil), rumen juice, urine, oil, industrial waste water, or lake water.

4. The analytical method for a liquid sample using near infrared spectroscopy according to claim 1, wherein a plurality of test tubes with the same specifications is used as a sample cell in the spectrum measurement of the sample for calibration.

5. An analytical apparatus for a liquid sample comprising:

a block provided with a housing portion for a test tube;

a near infrared apparatus provided with a spectroscope for dispersing near infrared light in a short wavelength range from light from a source of light or the sample and an optical sensor for detecting the near infrared light;

light conduction means for conducting the near infrared light in the short wavelength range emitted from the light source or the spectroscope to the test tube within the housing portion and for conducting, directly or through the spectroscope, diffusely reflected light, diffusely transmitted light, or transmitted and reflected light from the liquid sample within the test tube to the optical sensor; and

control means for outputting a measurement command of a spectrum to the near infrared apparatus and for replacing the measured spectrum with a value obtained from a calibration equation which has been made in advance, thereby computing object characteristics of the liquid sample.

6. The analytical apparatus for a liquid sample according to claim 5, wherein a white light source such as a tungsten halogen lamp is used as the light source, and a diode array is used as the optical sensor.

7. The analytical apparatus for a liquid sample according to claim 5, wherein the monochromatic near infrared light in a short wavelength range is used as the light source, and a silicon detector is used as the optical sensor.

8. The analytical apparatus for a liquid sample according to claim 5, wherein the light conduction means is an optical fiber or a bundle of the optical fibers.

9. The analytical apparatus for a liquid sample according to claim 5, wherein the block is provided with a temperature control means for stabilizing the liquid sample within the test tube at a predetermined temperature.