Application of bioinformatics for direct study of unculturable microorganisms
First Claim
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1. A method for identifying unculturable microorganisms comprising the steps of:
- isolating at least one bacterial cell from an environmental sample comprising a plurality of microorganisms;
amplifying at least one DNA fragment from said at least one bacterial cell;
cloning said at least one DNA fragment into at least one E. coli vector;
sequencing said at least one DNA fragment, resulting in identification of at least one DNA sequence; and
comparing said at least one DNA sequence with existing DNA databases, resulting in identification of said at least one DNA sequence as one of an unculturable microorganism and a known microorganism.
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Abstract
A method for identifying unculturable microorganisms in which at least one bacterial cell from an environmental sample containing a plurality of microorganisms is isolated, at least one DNA fragment from the at least one bacterial cell is amplified, cloned into at least one E. coli vector and sequenced, resulting in identification of at least one DNA sequence. The at least one DNA sequence is compared with existing DNA databases, resulting in identification of the at least one DNA sequence as derived from either an unculturable microorganism or a known microorganism.
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21 Claims
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1. A method for identifying unculturable microorganisms comprising the steps of:
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isolating at least one bacterial cell from an environmental sample comprising a plurality of microorganisms;
amplifying at least one DNA fragment from said at least one bacterial cell;
cloning said at least one DNA fragment into at least one E. coli vector;
sequencing said at least one DNA fragment, resulting in identification of at least one DNA sequence; and
comparing said at least one DNA sequence with existing DNA databases, resulting in identification of said at least one DNA sequence as one of an unculturable microorganism and a known microorganism. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification