Method for transfecting cells using a magnetic field
First Claim
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1. A method for transfecting a cell comprising the step of bringing a complex comprising one or more vectors and one or more magnetic particles in contact with a cell by applying a suitable magnetic field.
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Abstract
Described is a method for transfecting a cell comprising bringing a complex comprising vector(s) and magnetic particle(s) in contact with a cell by applying a magnetic field and methods of treatment using said method. Furthermore, described is such a complex as well as methods for making it. Finally, pharmaceutical compositions, uses of such complexes and a kit are described. The method described is particularly useful where automatizable high-throughput transfection is required for large scale screening processes.
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24 Claims
- 1. A method for transfecting a cell comprising the step of bringing a complex comprising one or more vectors and one or more magnetic particles in contact with a cell by applying a suitable magnetic field.
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15. A method for the preparation of a complex useful for transfecting a cell, said method comprising the step of linking together one or more vectors and one or more magnetic particles;
wherein said vector(s) are not naked DNA and not an adeno-associated virus.
Specification