Two-dimensional spectral imaging system
First Claim
1. A method of performing spectral imaging comprising:
- providing a sample having a spectral code;
generating a first image of the sample;
transmitting the first image through a first bandpass filter; and
sensing the first image with at least one sensor.
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Accused Products
Abstract
Improved devices, systems, and methods for sensing and/or identifying signals from within a signal detection region are well-suited for identification of spectral codes. Large numbers of independently identifiable spectral codes can be generated by quite small bodies, and a plurality of such bodies or probes may be present within a detection region. Simultaneously imaging of identifiable spectra from throughout the detection region allows the probes to be identified. As the identifiable spectra can be treated as being generated from a point source within a much larger detection field, a prism, diffractive grading, holographic transmissive grading, or the like can spectrally disperse the images of the labels across a sensor surface. A CCD can identify the relative wavelengths of signals making up the spectra. Absolute signal wavelengths may be identified by determining positions of the labels, by an internal wavelength reference within the spectra, or the like.
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Citations
47 Claims
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1. A method of performing spectral imaging comprising:
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providing a sample having a spectral code;
generating a first image of the sample;
transmitting the first image through a first bandpass filter; and
sensing the first image with at least one sensor. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 25, 26, 27, 28, 29, 30, 31)
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14. A method of performing spectral imaging comprising:
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providing a sample having a spectral code;
generating a first image of the sample;
bandpass filtering the image to transmit a first region of the electromagnetic spectrum;
generating a second image of the sample;
bandpass filtering the image to transmit a second region of the electromagnetic spectrum;
sensing the first image and second image with at least one sensor.
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23. A method of performing spectral imaging comprising:
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providing a sample having a spectral code;
generating an image of the sample;
splitting the image into at least a first image and a second image;
transmitting the first image through a first bandpass filter and the second image through a second bandpass filter; and
sensing the first image and the second image with at least one sensor.
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24. A method of optimizing an assay to determine an actual average measurement comprising:
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performing an assay on a set of beads;
measuring an assay signal from a first bead to obtain a first measurement;
measuring an assay signal from a second bead to obtain a second measurement;
calculating a candidate average measurement from the first measurement and the second measurement;
calculating a standard error from the first and second measurements;
comparing the standard error to a threshold value; and
determining that the candidate average measurement is the actual average measurement if the standard error is less than or equal to the threshold value.
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32. A method of optimizing an assay to determine an actual first-set average measurement and an actual second-set average measurement comprising:
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performing an assay on a first set of beads;
measuring an assay signal from a first bead of the first set of beads to obtain at a first first-set measurement;
measuring an assay signal from a second bead of the first set of beads to obtain at a second first-set measurement;
calculating a candidate first-set average measurement from the first and second first-set measurements;
calculating a first-set standard error from the first and second first-set measurements;
comparing the first-set standard error to a first-set threshold value; and
performing an assay on a second set of beads;
measuring an assay signal from a first bead of the second set of beads to obtain at a first second-set measurement;
measuring an assay signal from a second bead of the second set of beads to obtain at a second second-set measurement;
calculating a candidate second-set average measurement from the first and second second-set measurements;
calculating a second-set standard error from the first and second second-set measurements;
comparing the second-set standard error to a second-set threshold value; and
determining that the candidate first-set average measurement is the actual first-set average measurement and the candidate second-set average measurement is the actual second-set average measurement if the first-set standard error is less than or equal to the first-set threshold value and the second-set standard error is less than or equal to the second-set threshold value. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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Specification