Method and apparatus for the quantitative analysis of a liquid sample with surface enhanced spectroscopy

• US 20020093651A1
• Filed: 11/05/2001
• Published: 07/18/2002
• Est. Priority Date: 06/25/1998
• Status: Active Grant

• Current Assignee: Roche Diabetes Care Inc. (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Roe, Jeffrey N.
• Granted Patent: US 6,646,738 B2
• Time in Patent Office: Days
• US Class Current: 356/301
• CPC Class Codes: G01N 21/35 using infrared light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

Surface-enhanced spectroscopy for quantitative analysis of analytes in biological liquid samples. A device for testing a biological liquid sample for the presence or concentration of an analyte includes: (a) a substrate defining a surface which has electrically conductive particles disposed thereon, wherein the surface is adapted to accommodate the liquid sample; (b) means for generating radiation and directing the radiation to be incident on said surface of the substrate so that radiation is emitted from the electrically conductive particles; and (c) means for detecting the emitted radiation. In another embodiment a matrix having electrically conductive particles that are incorporated in the matrix or that are disposed on a surface thereof, wherein the matrix is permeable to the liquid biological sample is used in place of the substrate. The device is particularly suited for determining glucose in human whole blood.

Claims

1. A device for testing a biological liquid sample for the presence or concentration of an analyte comprising:

(a) a substrate defining a surface which has electrically conductive particles disposed thereon, wherein the surface is adapted to accommodate the liquid sample;

(b) means for generating radiation and directing the radiation to be incident on said surface of the substrate so that radiation is emitted from the electrically conductive particles; and

(c) means for detecting the emitted radiation.

2. The device of claim 1 wherein the detector means measures Raman scattered radiation.

3. The device of claim 2 wherein the radiation generated has a wavelength that ranges from about 300 nm to 1000 nm.

4. The device of claim 2 further comprising means to generate a spectral representation from the detected radiation.

5. The device of claim 2 wherein the radiation generation means comprises:

(i) a radiation emitting laser;

(ii) a fiber optic device having an input end that is coupled to the laser for delivering the radiation to irradiate a region of the substrate having a biological liquid sample thereon; and

(iii) a first optical filter having an input end that is coupled to a distal end of the fiber optic device that filters radiation and an output end for passing the laser radiation.

6. The device of claim 5 wherein the detection means comprises:

(i) an optical concentration that reflects radiation and an aperture at a distal end that collects radiation, including Raman-scattered radiation from biological liquid sample, and an output path at the proximal end coupled to the output end of the first filter; and

(ii) a fiber optic bundle having an input end that is coupled to a second optical filter for passing the Raman-scattered radiation and an output end for delivering the Raman-scattered radiation.

7. The device of claim 6 wherein the detector means is coupled to the output end of the fiber optic bundle for detecting the Raman-scattered radiation wherein the detector means further comprises a data processor that determines a concentration level of the analyte.

8. The device of claim 2 wherein the substrate comprises a metal film having metal particles on a surface of the film.

9. The device of claim 8 wherein the metal film comprises a metal that is selected from the group consisting of gold, silver, platinum copper, and mixtures thereof.

10. The device of claim 9 wherein the metal film has a nominal thickness that ranges from about 0.1 μ

m to 10 μ

m.

11. The device of claim 2 wherein the electrically conductive particles are selected from electrically conductive carbon and metal coated microspheres.

12. The device of claim 2 wherein the biological liquid sample is blood and the analyte is glucose.

13. The device of claim 1 wherein the radiation generating means generates infrared radiation having a wavelength such that infrared radiation absoprtion occurs in the presence of the analyte.

14. The device of claim 13 wherein the radiation generated has a wavelength that ranges from about 1 μ

m to 15 μ

m.

15. The device of claim 13 further comprising means to generate a spectral representation from the detected radiation.

16. The device of claim 13 wherein the radiation generating means includes a radiation source that is selected from a light emitting diode, laser diode, and tungsten halogen lamp.

17. The device of claim 16 further comprising a data processor that determines a concentration level of the analyte.

18. The device of claim 13 wherein the substrate comprises a metal film having metal particles on a surface of the film.

19. The device of claim 18 wherein the metal film comprises a metal that is selected from the group consisting of gold, silver, platinum, copper, and mixtures thereof.

20. The device of claim 19 wherein the metal film has a nominal thickness that ranges from about 0.1 nm to 10 nm.

21. The device of claim 13 wherein the electrically conductive particles are selected from electrically conductive carbon and metal coated microspheres.

22. The device of claim 11 wherein the biological liquid sample is blood and the analyte is glucose.

23. The device of claim 1 wherein the substrate is transparent to radiation directed to be incident on the substrate surface.

25. The device of claim 24 wherein the detector means measures Raman scattered radiation.

26. The device of claim 25 wherein the radiation generated has a wavelength that ranges from about 300 nm to 1000 nm.

27. The device of claim 25 further comprising means to generate a spectral representation from the detected radiation.

28. The device of claim 25 wherein the radiation generation means comprises:

(i) a radiation emitting laser;

(ii) a fiber optic device having an input end that is coupled to the laser for delivering the radiation to irradiate a region of the substrate having a biological liquid sample thereon; and

(iii) a first optical filter having an input end that is coupled to a distal end of the fiber optic device that filters radiation and an output end for passing the laser radiation.

29. The device of claim 28 wherein the detector means comprises:

(i) an optical concentration that reflects radiation and an aperture at a distal end that collects radiation, including Raman-scattered radiation from biological liquid sample, and an output path at the proximal end coupled to the output end of the first filter; and

(ii) a fiber optic bundle having an input end that is coupled to a second optical filter for passing the Raman-scattered radiation and an output end for delivering the Raman-scattered radiation.

30. The device of claim 29 wherein the detector means is coupled to the output end of the fiber optic bundle for detecting the Raman-scattered radiation wherein the detector means further comprises a data processor that determines a concentration level of the analyte.

31. The device of claim 25 wherein the substrate comprises a metal film having metal particles on a surface of the film.

32. The device of claim 31 wherein the metal particles comprises a metal that is selected from the group consisting of gold, silver, platinum, copper, and mixtures thereof.

33. The device of claim 32 wherein the metal particles have a size that ranges from about 0.1 μ

m to 10 μ

m.

34. The device of claim 25 wherein the electrically conductive particles are selected from electrically conductive carbon and metal coated microspheres.

35. The device of claim 25 wherein the biological liquid sample is blood and the analyte is glucose.

36. The device of claim 24 wherein the radiation generating means generates infrared radiation having a wavelength such that infrared radiation absoprtion occurs in the presence of the analyte.

37. The device of claim 36 wherein the radiation generated has a wavelength that ranges from about 1 μ

m to 15 μ

m.

38. The device of claim 36 further comprising means to generate a spectral representation from the detected radiation.

39. The device of claim 34 wherein the radiation generating means includes a radiation source that is selected from a light emitting diode, laser diode, and tungsten halogen lamp.

40. The device of claim 36 further comprising a data processor that determines a concentration level of the analyte.

41. The device of claim 37 wherein the substrate comprises a metal film having metal particles on a surface of the film.

42. The device of claim 41 wherein the metal film comprises a metal that is selected from the group consisting of gold, silver, platinum, copper, and mixtures thereof.

43. The device of claim 42 wherein the metal film has a nominal thickness that ranges from about 0.1 nm to 10 nm.

44. The device of claim 36 wherein the electrically conductive particles is selected from electrically conductive carbon and metal coated microspheres.

45. The device of claim 36 wherein the biological liquid sample is blood and the analyte is glucose.

46. The device of claim 24 wherein the substrate is transparent.

48. The method of claim 47 further comprising the step of collecting the emitted Raman-scattered radiation.

49. The method of claim 47 wherein the analyte is located adjacent to or chemisorbed onto the particles.

24. A device for testing a liquid biological sample for the presence or concentration of an analyte comprising:

(a) a matrix having electrically conductive particles that are incorporated in the matrix or that are disposed on a surface thereof, wherein the matrix is permeable to the liquid biological sample;

(b) means for generating radiation and directing the radiation to be incident on matrix so that radiation is emitted from the electrically conductive particles; and

(c) means for detecting radiation that is emitted from the metal particles.

47. A method of testing a liquid biological sample for the presence or concentration of an analyte that comprises the steps of:

(a) contacting the sample to electrically conductive particles to cause analytes present in the sample to be associated with the particles;

(b) irradiating the particles with radiation to cause Raman scattering to occur in the presence of the analyte; and

(c) detecting Raman-scattered radiation from the emitted analyte in response to the irradiation.

50. A method of testing a liquid biological sample for the presence or concentration of an analyte that comprises the steps of:

(a) contacting the sample to electrically conductive particles to cause analytes present in the sample to be associated with the particles;

(b) irradiating the particles with radiation having a wavelength such that infrared radiation absorption occurs in the presence of the analyte; and

(c) detecting radiation emitted from the particles in response to the irradiation.

51. The method of claim 50 further comprising the step of collecting the emitted radiation.

52. The method of claim 50 wherein the analyte is located adjacent to or is chemisorbed onto the particles.