Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof

• US 20020094541A1
• Filed: 05/21/2001
• Published: 07/18/2002
• Est. Priority Date: 02/17/2000
• Status: Active Grant

• Current Assignee: Glycominds Diagnostics Ltd.
• Original Assignee: Avinoam Dukler, Nir Dotan
• Inventors: Dotan, Nir, Dukler, Avinoam
• Granted Patent: US 6,994,966 B2
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07H 15/02 Acyclic radicals, not substituted by cyclic structures
• CPC Class Codes: C07H 15/203 Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
• CPC Class Codes: C07K 14/59 Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
• CPC Class Codes: C40B 30/04 by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
• CPC Class Codes: G01N 2333/42 Lectins, e.g. concanavalin, phytohaemagglutinin
• CPC Class Codes: G01N 2400/02 involving antibodies to sugar part of glycoproteins lectins from plants G01N2333/42, lectins from mammals G01N2333/4724
• CPC Class Codes: G01N 2400/10 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: G01N 33/5308 for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose
• CPC Class Codes: G01N 33/6842 Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
• CPC Class Codes: G01N 33/6845 Methods of identifying protein-protein interactions in protein mixtures

Abstract

A combinatorial complex carbohydrate library is provided and including a plurality of addressable complex carbohydrate structures.

Claims

1. A method of identifying a complex carbohydrate capable of binding an entity, the method comprising the steps of:

(a) producing an addressable combinatorial complex carbohydrate library by;

(i) providing a solid support having a plurality of locations; and

(ii) enzymatically synthesizing a plurality of complex carbohydrate structures, each of said plurality of complex carbohydrate structures being attached to at least one addressed location of said plurality of locations, thereby producing the addressable combinatorial complex carbohydrate library; and

(b) screening said addressable combinatorial complex carbohydrate library with the entity for identifying the complex carbohydrate capable of binding the entity.

2. The method of claim 1, wherein said entity is selected from the group consisting of proteins encoded by an EST library, and proteins extracted from a natural source.

3. The method of claim 1, wherein the entity is a candidate for a biologically active material, the method serves for identifying a complex carbohydrate which is a target for said candidate for a biologically active material.

4. The method of claim 1, wherein the entity is a ligand known to bind a specific natural complex carbohydrate and further wherein said addressable combinatorial complex carbohydrate library is a representation of domains of said specific natural complex carbohydrate, the method serves for identifying a specific domain of said domains which binds said ligand.