Biochannel assay for hybridization with biomaterial

• US 20020094584A1
• Filed: 12/19/2001
• Published: 07/18/2002
• Est. Priority Date: 11/12/1999
• Status: Active Grant

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Motorola, Inc. (Motorola Solutions, Inc.)
• Inventors: Shieh, Chan-Long, Yu, Huinan, Choong, Vi-En, Foley, Barbara
• Granted Patent: US 6,960,467 B2
• Time in Patent Office: Days
• US Class Current: 436/514
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00351 Means for dispensing and evacuation of reagents
• CPC Class Codes: B01J 2219/00416 Vacuum
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00421 using centrifugation
• CPC Class Codes: B01J 2219/00432 Photolithographic masks
• CPC Class Codes: B01J 2219/0052 in the shape of elongated tubes
• CPC Class Codes: B01J 2219/00522 in a multiple parallel arrangement
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01L 2300/0636 : Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0816 : Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/087 : Multiple sequential chambers
• CPC Class Codes: B01L 2400/0415 : electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/043 : magnetic forces
• CPC Class Codes: B01L 2400/049 : vacuum
• CPC Class Codes: B01L 3/5027 : by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 : characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 7/525 : with physical movement of samples between temperature zones
• CPC Class Codes: B82Y 30/00 : Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6834 : Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/629 : being a microfluidic device
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: Y10S 366/02 : Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway
• CPC Class Codes: Y10S 435/81 : Packaged device or kit

Abstract

The invention relates to a microfluidic device with microchannels that have separated regions which have a member of a specific binding pair member such as DNA or RNA bound to porous polymer, beads or structures fabricated into the microchannel. The microchannels of the invention are fabricated from plastic and are operatively associated with a fluid propelling component and detector.

Claims

1. A microfluidic device comprising a section of solid material with a microchannel having an entrance and exit port for the transportation of fluids in and out of the microchannel wherein the microchannel contains spacially separated defined regions of specific binding pair member immobilized on a porous polymer, beads or on microstructures fabricated in the microchannel.

2. The microfluidic device of claim 1 wherein the device is fabricated from silicon, glass, silicon dioxide, plastic or ceramics.

3. The microfluidic device of claim 1 wherein the separated defined regions are porous polymer with specific binding pair member bound to the porous polymer.

4. The microfluidic device of claim 1 wherein the separated defined regions have beads with specific binding pair member bound to the bead.

5. The microfluidic device of claim 1 wherein the defined regions are with immobilized binding pair members are formed by introducing hydrogels in the microchannels.

6. The defined region of claim 5 wherein the binding pair members are selectively dispensed on spatially separated portions of hydrogel.

7. The defined region of claim 5 wherein the hydrogels in the microchannels are patterned by means including photolithography.

8. The microfluidic device of claim 1 wherein the separated defined regions have microstructures fabricated into the microchannel and the microstructures have specific binding pair member bound thereto.

9. The microfluidic device of claim 1 wherein the binding pair members from a group consisting of DNA, RNA, polypeptides, nucleic acids, and antibody/antigens.

10. The microfluidic device of claim 1 wherein the specific binding member is a DNA or RNA probe.

11. The microfluidic device of claim 1 wherein the specific binding member is DNA.

12. The microfluidic device of claim 1, further comprising a fluid propelling component that is operatively associated with the microchannels.

13. The fluid propelling component in claim 12 is a pressurized gas, vacuum, electrical field, magnetic field or centrifugal force.

14. The microfluidic device of claim 1, comprising a detector component that is operatively associated with the microchannels.

15. The microfluidic device of claim 14 wherein the detector is an optical, electrical or electrochemical detector.

16. A method of detecting a specific binding member in a test sample comprising:

a. providing the microfluidic device of claim 1;

b. flowing the test sample through the microchannel to form a binding pair;

c. detecting the binding pair.

17. The method of claim 16 wherein the flow of the test sample is recirculated in the microchannel.

18. The method of claim 16 wherein the flow rate of the test sample is adjusted by a fluid propelling component operatively associated with the microchannel.

19. The method of claim 16 wherein the migration speed of a charged sample is further modified through applying a modular electrical field in or against the direction of flow.

20. The method of claim 16 wherein the charged test sample is attracted or repelled at the spatially defined region by the application of electrical field in the direction perpendicular to the flow direction.