Novel human polynucleotides and polypeptides encoded thereby

• US 20020095031A1
• Filed: 05/03/2000
• Published: 07/18/2002
• Est. Priority Date: 05/04/1999
• Status: Abandoned Application

• Current Assignee: Lexicon Genetics, Incorporated
• Original Assignee: Lexicon Genetics, Incorporated
• Inventors: Sands, Arthur T., Zambrowicz, Brian, Nehls, Michael C.
• Application Number:
  US09/563,817
• Publication Number:
  US 20020095031A1
• Time in Patent Office: Days
• US Class Current: 536/23.1
• CPC Class Codes: C12N 15/11 DNA or RNA fragments; Modified forms thereof DNA or RNA not used in recombinant technology, C07H21/00; Non-coding nucleic acids having a biological activity
• CPC Class Codes: G16B 30/00 ICT specially adapted for sequence analysis involving nucleotides or amino acids
• CPC Class Codes: G16B 30/10 Sequence alignment; Homology search

Abstract

Novel human polynucleotides are disclosed that correspond to human gene trapped sequences, or GTSs. The disclosed GTSs are useful for gene discovery and as markers for, inter alia, gene expression analysis, identifying and mapping the coding regions of the mammalian, and particularly human, genome, forensic analysis, and determining the genetic basis of human disease.

Claims

1. An isolated or purified polynucleotide comprising the sense or antisense sequence of any one of SEQ ID NOS:

9-1,008 or a fragment of at least 8 contiguous nucleotides first disclosed in any one of SEQ ID NOS;

9-1,008.

2. An isolated or purified gene or cDNA that hybridizes under stringent conditions to the sense or antisense sequence of any of SEQ ID NOS:

9-1,008 or a fragment at least 8 contiguous nucleotides first disclosed in any one of SEQ ID NOS;

9-1,008.

3. The isolated or purified polynucleotide of claim 2, wherein the fragment is at least 60 nucleotides.

4. A recombinant expression vector comprising the polynucleotide of claim 3.

5. A host cell comprising the vector of claim 4.

6. An in vitro process for producing a polynucleotide comprising the steps of:

a) obtaining a polynucleotide template encoding a sequence capable of hybridizing to a GTS of any one of SEQ ID NOS;

9-1,008;

b) combining said template with a synthetic oligonucleotide sequence of about 14 to about 80 bases in length that comprises a contiguous sequence of at least about 12 nucleotides first disclosed in any one of SEQ ID NOS;

9-1,008; and

c) processing the combined oligonucleotide and template preparation such that said oligonucleotide sequence hybridizes to said template in the presence of a DNA polymerase molecule and a sufficient concentration of dNTPs for said oligonucleotide sequence to prime DNA synthesis by said polymerase,

wherein a polynucleotide is produced that encodes at least about 50 contiguous nucleotides in any one of SEQ ID NOS;

9-1,008.

7. The process of claim 6 wherein said template is mammalian cDNA.

8. A process according to claim 7 wherein said template is of human origin.

9. The process of claim 6 wherein said template is mammalian genomic DNA.

10. A process according to claim 9 wherein said template is of human origin.

11. A computer readable medium having recorded thereon the sense or antisense sequence of any one of SEQ ID NOS:

9-1,008 or a fragment at least 8 contiguous nucleotides first disclosed therein or a nucleotide sequence at least 99% identical thereto.

12. The computer readable medium of claim 11, wherein said medium is selected from the group consisting of a floppy disc, a hard disc, random access memory (RAM), read only memory (ROM), and CD-ROM.

13. A computer-based system for identifying nucleic acid fragments of the human genome of commercial importance comprising the following elements:

a) a data storage means comprising the sense or antisense sequence of any one of SEQ ID NOS;

9-1,008 or a fragment at least 8 contiguous nucleotides first disclosed therein or a nucleotide sequence at least 99% identical thereto;

b) search means for comparing a target sequence to the nucleotide sequence of the data storage means of step a) to identify homologous sequence(s); and

c) retrieval means for obtaining said homologous sequence(s) of step (b).