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Amplification based polymorphism detection

  • US 20020102549A1
  • Filed: 12/21/2000
  • Published: 08/01/2002
  • Est. Priority Date: 12/30/1999
  • Status: Active Grant
First Claim
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1. A method for distinguishing between the presence of a target nucleic acid sequence and a variant of the target nucleic acid sequence in a test sample, wherein the variant nucleic acid contains a variation from the target nucleic acid sequence comprising a deletion of eight or more consecutive nucleotides, the method comprises the steps of:

  • a) contacting the test sample with amplification reagents and a first and second amplification primer to form a reaction mixture, wherein the first primer hybridizes with the target nucleic acid sequence at the site containing the variation in the variant sequence;

    b) subjecting the reaction mixture to amplification conditions; and

    c) detecting the presence of an amplification product from the first and second primers as an indication of the presence of the target nucleic acid sequence in the test sample.

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