Assays and probes with enzyme labels
First Claim
Patent Images
1. A probe for use in an assay comprising a nuclease selected from P1 and S1 nucleases coupled to a specific binding member (“
- sbm”
).
0 Assignments
0 Petitions
Accused Products
Abstract
Probes comprise S1 and P1 nuclease (as an enzyme label) linked to a specific binding member such as a nucleotide sequence or an antibody. Such probes are useful for sandwich assays. As compared with known probes using alkaline phosphatase as a label, advantages include relative insensitivity to phosphate and elevated temperature and reduced risk of nonspecific binding.
7 Citations
17 Claims
- 1. A probe for use in an assay comprising a nuclease selected from P1 and S1 nucleases coupled to a specific binding member (“
Specification