49937, 49931, and 49933, novel human transporter family members and uses thereof
First Claim
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1. An isolated nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO;
1, 5, or 8; and
(b) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO;
3, 7, or 10.
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Accused Products
Abstract
The invention provides isolated nucleic acid molecules, designated HEAT nucleic acid molecules, which encode novel transporter family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing HEAT nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a HEAT gene has been introduced or disrupted. The invention still further provides isolated HEAT proteins, fusion proteins, antigenic peptides and anti-HEAT antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.
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Citations
31 Claims
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1. An isolated nucleic acid molecule selected from the group consisting of:
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(a) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO;
1, 5, or 8; and
(b) a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO;
3, 7, or 10. - View Dependent Claims (6, 7, 8, 9, 10, 11, 12, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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2. An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2, 6, or 9.
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3. An isolated nucleic acid molecule comprising the nucleotide sequence contained in the plasmid deposited with ATCC®
- as Accession Number ______, ______, or ______.
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4. An isolated nucleic acid molecule which encodes a naturally-occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:
- 2, 6, or 9.
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5. An isolated nucleic acid molecule selected from the group consisting of:
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(a) a nucleic acid molecule comprising a nucleotide sequence which is at least 60% identical to the nucleotide sequence of SEQ ID NO;
1, 3, 5, 7, 8, or 10, or a complement thereof;
(b) a nucleic acid molecule comprising a fragment of at least 30 nucleotides of a nucleic acid comprising the nucleotide sequence of SEQ ID NO;
1, 3, 5, 7, 8, or 10, or a complement thereof;
(c) a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence at least about 60% identical to the amino acid sequence of SEQ ID NO;
2, 6, or 9; and
(d) a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO;
2, 6, or 9, wherein the fragment comprises at least 10 contiguous amino acid residues of the amino acid sequence of SEQ ID NO;
2, 6, or 9.
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13. An isolated polypeptide selected from the group consisting of:
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a) a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO;
2, 6, or 9, wherein the fragment comprises at least 10 contiguous amino acids of SEQ ID NO;
2, 6, or 9;
b) a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO;
2, 6, or 9, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to complement of a nucleic acid molecule consisting of SEQ ID NO;
1, 3, 5, 7, 8, or 10 under stringent conditions;
c) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 60% identical to a nucleic acid comprising the nucleotide sequence of SEQ ID NO;
1, 3, 5, 7, 8, or 10; and
d) a polypeptide comprising an amino acid sequence which is at least 60% identical to the amino acid sequence of SEQ ID NO;
2, 6, or 9.
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27. A method of identifying a subject having a cardiovascular disorder, or at risk for developing a cardiovascular disorder comprising:
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a) contacting a sample obtained from said subject comprising nucleic acid molecules with a hybridization probe comprising at least 25 contiguous nucleotides of SEQ ID NO;
1, 5, or 8; and
b) detecting the presence of a nucleic acid molecule in said sample that hybridizes to said probe, thereby identifying a subject having a cardiovascular disorder.
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28. A method of identifying a subject having a cardiovascular disorder, or at risk for developing a cardiovascular disorder comprising:
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a) contacting a sample obtained from said subject comprising nucleic acid molecules with a first and a second amplification primer, said first primer comprising at least 25 contiguous nucleotides of SEQ ID NO;
1, 5, or 8 and said second primer comprising at least 25 contiguous nucleotides from the complement of SEQ ID NO;
1, 5, or 8;
b) incubating said sample under conditions that allow nucleic acid amplification; and
c) detecting the presence of a nucleic acid molecule in said sample that is amplified, thereby identifying a subject having a cardiovascular disorder, or at risk for developing a cardiovascular disorder.
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29. A method of identifying a subject having a cardiovascular disorder, or at risk for developing a cardiovascular comprising:
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a) contacting a sample obtained from said subject comprising polypeptides with a HEAT binding substance; and
b) detecting the presence of a polypeptide in said sample that binds to said HEAT binding substance, thereby identifying a subject having a cardiovascular disorder, or at risk for developing a cardiovascular disorder.
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30. A method for identifying a compound capable of treating a cardiovascular disorder characterized by aberrant HEAT nucleic acid expression or HEAT polypeptide activity comprising assaying the ability of the compound to modulate HEAT nucleic acid expression or HEAT polypeptide activity, thereby identifying a compound capable of treating a cardiovascular disorder characterized by aberrant HEAT nucleic acid expression or HEAT polypeptide activity.
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31. A method for treating a subject having a cardiovascular disorder characterized by aberrant HEAT polypeptide activity or aberrant HEAT nucleic acid expression comprising administering to the subject a HEAT modulator, thereby treating said subject having a cardiovascular disorder.
Specification