Apparatus and method for electrophoresis
First Claim
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1. Apparatus for conducting electrophoresis, said apparatus comprising:
- a chamber defining an electrophoresis area, said area comprising a gel matrix, said chamber further comprising a sealed region;
an anode within said chamber and in contact with said gel matrix, wherein said anode is contained within said sealed region; and
a cathode within said chamber and in contact with said gel matrix.
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Abstract
Apparatus for conducting electrophoresis therein includes a chamber with a gel matrix. The chamber has a first sealed region and a second sealed region, and an anode within the first sealed region of the chamber and in contact with the gel matrix, and a cathode within the second sealed region and in contact with the gel matrix. At least one of the electrodes also provides ions for driving the electrophoresis. The apparatus further includes a matrix with at least one sparingly water-soluble salt.
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Citations
78 Claims
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1. Apparatus for conducting electrophoresis, said apparatus comprising:
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a chamber defining an electrophoresis area, said area comprising a gel matrix, said chamber further comprising a sealed region;
an anode within said chamber and in contact with said gel matrix, wherein said anode is contained within said sealed region; and
a cathode within said chamber and in contact with said gel matrix. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 28, 29, 30, 31, 32, 33, 34, 36, 37, 38, 39, 40, 41, 42, 43, 44, 46, 47, 48, 49, 50, 51, 52)
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16. Apparatus for conducting electrophoresis, said apparatus comprising:
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a chamber defining an electrophoresis area, said area comprising a gel matrix, said chamber further comprising a sealed region;
a cathode within said chamber and in contact with said gel matrix, wherein said cathode is contained within said sealed region of the chamber; and
an anode within said chamber and in contact with said gel matrix.
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27. Apparatus for conducting electrophoresis, said apparatus comprising:
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a chamber defining an electrophoresis area, said area comprising a gel matrix, said chamber further comprising a first sealed region and a second sealed region;
an anode with said chamber and in contact with said gel matrix, wherein said anode is contained within said first sealed region; and
a cathode within said chamber and in contact with said gel matrix, wherein said cathode is contained within said second sealed region.
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35. The apparatus of clam 27, wherein said cathode comprises hydrogen-absorbing material.
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45. An apparatus for conducting electrophoresis the apparatus comprising:
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a chamber defining an electrophoresis area, said electrophoresis area having at least one body of gel matrix for facilitating said electrophoresis;
a first electrode; and
a second electrode, wherein said fist electrode is an anode, wherein said second electrode is a cathode, and wherein said first electrode and said second electrode are each in contact with said chamber, at least one of said first electrode and said second electrode is embedded within said at least one body of gel matrix, and wherein said at least one body of gel matrix comprises ions located within said gel matrix, said ions generated during an electrochemical reaction of said first electrode.
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53. An apparatus for conducting electrophoresis the apparatus comprising:
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a chamber defining an electrophoresis area, said electrophoresis area having at least one body of a gel matrix for facilitating said electrophoresis;
a first electrode; and
a second electrode, wherein said first electrode and said second electrode are in contact with said chamber, and at least one of said first electrode and said second electrode is embedded within said at least one body of the gel matrix, wherein said first electrode is an anode and said second electrode is a cathode, said at least one body of said gel matrix comprises electrolyte solution, said anode comprises an electrochemically ionizable metal, and said electrolyte solution is of a composition such that migration of ions generated during an electrochemical reaction at said anode is inhibited, wherein said electrochemical reaction is not water electrolysis. - View Dependent Claims (54, 55, 56, 57, 58, 60, 61)
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59. The apparatus of clam 58, wherein sad apertures corresponding to said loading sites are arranged in one or more rows.
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62. An apparatus for conducting electrophoresis the apparatus comprising:
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a chamber defining an electrophoresis area, said chamber comprising a sealed region, said electrophoresis area comprising at least one body of gel matrix for facilitating said electrophoresis;
a first electrode; and
a second electrode, wherein said first and said second electrodes are in contact with said chamber, at least one of said first and said second electrodes is embedded within said at least one body of gel matrix, said first electrode is an anode and said second electrode is a cathode, said at least one body of said gel matrix comprises electrolyte solution, said anode is contained within said sealed region and comprises an electrochemically ionizable metal, and said electrolyte solution is of a composition such that migration of ions generated during an electrochemical reaction at said anode is inhibited, wherein said electrochemical reaction is not water electrolysis. - View Dependent Claims (63, 64, 65, 66, 67, 68, 69, 70)
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71. A method for electrophoresis, the method comprising the steps of:
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applying an electrical field to a gel;
degrading a metal anode by said application of said electrical field; and
releasing ions required for maintaining an electrical field by said degradation. - View Dependent Claims (72)
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73. A method for electrophoresis, the method comprising the steps of;
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applying au electrical field to a gel;
degrading a sparingly water-soluble salt in contact with a cathode by said application of said electrical field; and
releasing ions required for maintaining an electrical field by said degradation. - View Dependent Claims (74)
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75. A method for electrophoresis, the method comprising the steps of:
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applying an electrical field to a gel;
degrading a metal anode by said application of said electrical field;
degrading a sparingly water-soluble salt in contact with a cathode by said application of said electrical field; and
releasing ions required for maintaining au electrical field by said degradations. - View Dependent Claims (76)
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77. A method for electrophoresis, the method comprising the steps of:
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applying an electrical field to a gel;
degrading a metal anode by said application of said electrical field;
releasing ions required for maintaining an electrical field by said degradation, and inhibiting migration of said ions in the vicinity of said anode. - View Dependent Claims (78)
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Specification