Clam shell UF centrifugal filter vessel and method
First Claim
1. A method of forming an ultrafiltration vessel, such method comprising the steps of arranging two sheets of filter material skin-to-skin between vessel halves and overmolding a compressive body around the vessel halves to form a vessel under conditions effective to seal the sheets against each other along edges thereof.
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Accused Products
Abstract
A clamshell fabrication process wherein an ultrafiltration (UF) vessel (or a strip or array of filter vessels) is formed using half cells joined along a central plane to form each vessel. A filter membrane covers a port of the cell, and an outer body is overmolded to form the completed vessel. Two filter membranes are crushed together skin to skin. When applied to strips of half cells, a multi-well strip or array is formed, with sample-holding reservoirs formed by the space created between two strips of half-cells for each row of filter cells. The membrane thus covers the entire wetted interior of each cell, and may extend from near the opening at the top of an open cell to a conical bottom of the cell. A number of strip assemblies can be formed together to create larger arrays of filter cells. The overmolding process creates a crush seal with edges of the filter membrane. The vessel may have a regenerated surface, or be surface treated to be non-retentive, further enhancing yield when used to process sticky or adherent materials, such as proteins and biomolecules. This provides quantitative yields of filtrate, and retentate.
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Citations
12 Claims
- 1. A method of forming an ultrafiltration vessel, such method comprising the steps of arranging two sheets of filter material skin-to-skin between vessel halves and overmolding a compressive body around the vessel halves to form a vessel under conditions effective to seal the sheets against each other along edges thereof.
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5. A method of forming an ultrafiltration vessel, such method comprising the step of arranging one sheet of filter material between a first vessel part having at least one port, and a second vessel part such that the filter material covers the at least one port, and overmolding a body around the first and second vessel parts to form a vessel under conditions effective to seal the sheet along edges thereof between the first and second vessel parts.
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6. An ultrafiltration vessel, comprising
a vessel having an interior wall with an outlet port through the wall, and an ultrafiltration membrane covering the outlet port, wherein the interior wall has a regenerated cellulosic surface effective to minimize adsorption on said wall of material that passes through the filter to the port, thereby enhancing quantitative recovery of filtrate.
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7. An ultrafiltration vessel, comprising
a vessel having an interior wall with an outlet port through the wall, and an ultrafiltration membrane covering the outlet port, the ultrafiltration membrane having a skin-to-skin seal effective to cover a full area of the vessel wall with said filter.
Specification