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Gender differentiation of bovine sperm cells

  • US 20020115055A1
  • Filed: 09/27/2001
  • Published: 08/22/2002
  • Est. Priority Date: 09/28/2000
  • Status: Abandoned Application
First Claim
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1. A method for gender differentiation of bovine spermatozoa wherein monoclonal antibodies against male specific antigen, 17.18 kDa protein, associated with the classical pathway of the complement system are used after the inactivation of the alternative complement pathway, comprising:

  • a. incubating heated guinea pig serum with sperm cells and monoclonal antibodies, said monoclonal antibodies being specific enough to recognize only male specific antigens, and b. selectively rupturing the male sperm cells, leaving a majority of the live sperm cells as female.

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