METHODS FOR SELECTIVELY STIMULATING PROLIFERATION OF T CELLS
First Claim
1. A method for inducing a population of T cells to proliferate, comprising:
- a) activating a population of T cells;
b) stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule, the activating and stimulating steps thereby inducing proliferation of the T cells;
c) monitoring proliferation of the T cells in response to continuing exposure to the ligand; and
d) reactivating and restimulating the T cells when the rate of T cell proliferation has decreased to induce further proliferation of the T cells.
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Abstract
Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.
64 Citations
1 Claim
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1. A method for inducing a population of T cells to proliferate, comprising:
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a) activating a population of T cells;
b) stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule, the activating and stimulating steps thereby inducing proliferation of the T cells;
c) monitoring proliferation of the T cells in response to continuing exposure to the ligand; and
d) reactivating and restimulating the T cells when the rate of T cell proliferation has decreased to induce further proliferation of the T cells.
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Specification