Microdevices for high-throughput screening of biomolecules
First Claim
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1. A device for processing a fluid sample, having a plurality of noncontiguous reactive sites, each of said sites comprising:
- (a) a substrate;
(b) an immobilized biological moiety;
(b) a monolayer chemisorbed or physisorbed on a portion of a surface of the substrate, said monolayer comprising molecules of the formulaX—
R—
Ywherein R is a spacer, X is a functional group that binds R to the surface, and Y is a functional group for binding said biological moiety onto the monolayer; and
(c) an affinity tag, wherein said affinity tag enhances site-specific immobilization of said biological moiety onto the monolayer. wherein each of said sites may independently react with a component of the fluid sample and are separated from each other by a region of said substrate that is free of molecules of the formula X—
R—
Y.
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Abstract
Methods and devices for the parallel, in vitro screening of biomolecular activity using miniaturized microfabricated devices are provided. The biomolecules that can be immobilized on the surface of the devices of the present invention include proteins, polypeptides, nucleic acids, polysaccharides, phospolipids, and related unnatural polymers of biological relevance. These devices are useful in high-throughput drug screening and clinical diagnostics and are preferably used for the parallel screening of families of related proteins.
127 Citations
56 Claims
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1. A device for processing a fluid sample, having a plurality of noncontiguous reactive sites, each of said sites comprising:
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(a) a substrate;
(b) an immobilized biological moiety;
(b) a monolayer chemisorbed or physisorbed on a portion of a surface of the substrate, said monolayer comprising molecules of the formula X—
R—
Ywherein R is a spacer, X is a functional group that binds R to the surface, and Y is a functional group for binding said biological moiety onto the monolayer; and
(c) an affinity tag, wherein said affinity tag enhances site-specific immobilization of said biological moiety onto the monolayer. wherein each of said sites may independently react with a component of the fluid sample and are separated from each other by a region of said substrate that is free of molecules of the formula X—
R—
Y. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 50, 51, 52, 53, 54, 55, 56)
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49. A device for processing a fluid sample, comprising:
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(a) a substrate;
(b) a plurality of parallel microchannels microfabricated into or onto said substrate; and
(c) a biological moiety immobilized within at least one of said parallel microchannels, wherein said biological moiety may interact with a component of the fluid sample.
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Specification