Phosphatonin-related gene and methods of use thereof
First Claim
1. A method of modulating phosphate homeostasis in a subject comprising altering the activity of a polypeptide encoded by the FRP-4 gene within the subject.
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Abstract
This invention provides methods for modulating phosphate homeostasis and renal phosphate transport by delivering agents that alter the expression of the FRP-4 gene or alter the activity of the FRP-4 protein. The methods of the invention are useful for modulating bone mineralization, renal phosphate transport, alleviating oncogenic osteomalacia-associated symptoms and treating phosphate homeostasis-related disease. The invention further provides methods for reducing phosphate re-absorption by delivering to a subject FRP-4 protein or polynucleotides that encode this protein. In addition, the invention provides methods for detecting and monitoring expression of the FRP-4 gene and modulating the phenotype of a neoplastic cell associated with oncogenic osteomalacia. Finally, the invention provides methods for screening candidate agents to identify compositions that modify the activity of the FRP-4 gene and protein.
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Citations
14 Claims
- 1. A method of modulating phosphate homeostasis in a subject comprising altering the activity of a polypeptide encoded by the FRP-4 gene within the subject.
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3. A method of modulating phosphate homeostasis in a subject comprising altering the expression of a polynucleotide encoding FRP-4 polypeptide within the subject.
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4. A method for modulating renal phosphate transport in a subject, comprising delivering to the subject an effective amount of an agent that alters the activity of a polypeptide encoded by the FRP-4 gene.
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5. A method of reducing phosphate re-absorption in a subject comprising delivering to the subject an effective amount of the FRP-4 protein.
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6. A method of reducing phosphate re-absorption in a subject comprising delivering to the subject an effective amount of a polynucleotide encoding the FRP-4 protein.
- 7. A method of screening for candidate therapeutic agents that modulate the expression of the FRP-4 gene comprising contacting a target cell with a test agent and monitoring expression of the FRP-4 gene, wherein a test agent which modifies the expression of the FRP-4 gene is a candidate therapeutic agent.
- 9. A method of screening for candidate agents capable of altering the biological activity of a polypeptide encoded by the FRP-4 gene, comprising contacting a target cell expressing a FRP-4 polypeptide with a test agent and monitoring activity of the expressed polypeptide product, wherein a test agent which modifies the activity of the polypeptide is a candidate agent.
- 11. A method of screening for candidate agents that modulate the activity of the FRP-4 protein comprising contacting a target cell with a candidate agent and monitoring the activity of the FRP-4 protein, wherein a candidate agent which modifies the activity of the FRP-4 protein is a candidate therapeutic agent.
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13. A method of screening for candidate ligand that modulate the activity of the FRP-4 protein comprising contacting a target cell with a candidate agent and monitoring the activity of the FRP-4 protein, wherein a candidate agent which modifies the activity of the FRP-4 protein is a candidate ligand.
Specification