Antimicrobial peroxidase compositions

• US 20020119136A1
• Filed: 03/23/2001
• Published: 08/29/2002
• Est. Priority Date: 05/09/1996
• Status: Abandoned Application

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novozymes A/S (Novozymes)
• Inventors: Johansen, Charlotte
• Application Number:
  US09/815,848
• Publication Number:
  US 20020119136A1
• Time in Patent Office: Days
• US Class Current: 424/94.4
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 37/40 having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
• CPC Class Codes: A01N 43/78 1,3-Thiazoles; Hydrogenated 1,3-thiazoles
• CPC Class Codes: A01N 43/84 six-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,4
• CPC Class Codes: A01N 59/00 Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
• CPC Class Codes: A01N 59/12 Iodine, e.g. iodophors; Compounds thereof
• CPC Class Codes: A01N 59/24 Cyanogen or compounds thereof, e.g. hydrogen cyanide, cyanic acid, cyanamide, thiocyanic acid
• CPC Class Codes: A01N 61/00 Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: A01N 63/50 Isolated enzymes; Isolated proteins peptides A01N37/46
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: C11D 3/38654 containing oxidase or reductase
• CPC Class Codes: C11D 3/48 Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

Abstract

Enzymatic compositions comprising a Coprinus peroxidase, hydrogen, peroxide or a source of hydrogen peroxide, and an enhancing agent such as an electron donor, e.g., phenothiazine-10-propionic acid; 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate); acetosyringate; C_1-8-alkylsyringate; or a water-soluble halide or thiocynate salt such as potassium iodide, have antimicrobial properties useful e.g., for inhibiting or killing microorganizms present in laundry, on human or animal skin, hair, mucous membranes, oral cavities, teeth, wounds, bruises, and on hard surfaces, and can be used as disinfectant, a preservative for cosmetics, and for cleaning, disinfecting or inhibiting microbial growth on process equipment used for e.g. water treatment, food processing, chemical or pharmaceutical processing, paper pulp processing, and water sanitation.

Claims

1. A method of killing or inhibiting a microorganism, comprising contacting said microorganism with a composition comprising:

(a) a peroxidase produced by or derived from the fungus Coprinus;

(b) an enhancing agent; and

(c) a hydrogen peroxide or a source of hydrogen peroxide.

2. The method of claim 1, wherein the peroxidase is a recombinant enzyme obtainable from Coprinus cinereus.

3. The method of claim 1, wherein the peroxidase is obtainable from Coprinus cinereus, IFO 8371.

4. The method of claim 1, wherein the source of hydrogen peroxide is an enzymatic hydrogen peroxide-generating system.

5. The method of claim 4, wherein the enzymatic system is selected from the group consisting of glucose oxidase/glucose, hexose oxidase/hexose, L- or D-amino acid oxidase/L- or D-amino acid, and lactate oxidase/lactate.

6. The method of claim 1, wherein the enhancing agent is an electron donor.

7. The method of claim 1, wherein the enhancing agent is a water-soluble halide or thiocyanate salt.

8. The method of claim 1, wherein the enhancing agent is a compound having the formula:

9. The method of claim 1, wherein the enhancing agent is selected from the group consisting of 10-methylphenothiazine, phenothiazine-10-propionic acid, N-hydroxysuccinimide phenothiazine-10-propionate, 10-ethyl-phenothiazine-4-carboxylic acid, 10-ethylphenothiazine, lo-propylphenothiazine, 10-isopropylphenothiazine, methyl phenothiazine-10-propionate, 10-phenylphenothiazine, 10-allylphenothiazine, 10-(3-(4-methylpiperazin-1-yl)propyl)phenothiazine, 10-(2-pyrrolidin-1-yl-ethyl)phenothiazine, 2-methoxy-10-methyl-phenothiazine, 1-methoxy-10-methylphenothiazine, 3-methoxy-10-methylphenothiazine, 3,10-dimethylphenothiazine, 3,7,10-trimethylphenothiazine, 10-(2-hydroxyethyl)phenothiazine, 10-(3-hydroxypropyl)phenothiazine, 3-(2-hydroxyethyl)-10-methylphenothiazine, 3-hydroxymethyl-10-methylphenothiazine, 3,7-dibromophenothiazine-10-propionic acid, phenothiazine-10-propionamide, chlorpromazine, 2-chloro-10-methylphenothiazine, 2-acetyl-10-methylphenothiazine, 10-methylphenoxazine, 10-ethyl-phenoxazine, phenoxazine-10-propionic acid, 10-(2-hydroxyethyl)phenoxazine and 4-carboxyphenoxazine-10-propionic acid.

10. The method of claim 1, wherein the enhancing agent is a compound having the formula:

11. The method of claim 1, wherein the enhancing agent is acetosyringone, methylsyringate, ethylsyringate, propylsyringate, butylsyringate, hexylsyringate, or octylsyringate.

12. The method of claim 1, wherein the enhancing agent is a salt selected from the group consisting of potassium halide, sodium halide, lithium halide, ammonium halide, calcium halide.

13. The method of claim 1, wherein the enhancing agent is a salt salt selected from the group consisting of potassium iodide, sodium iodide, lithium iodide, ammonium iodide, and calcium iodide.

14. The method of claim 1, wherein the enhancing agent is a salt selected from the group consisting of sodium thiocyanate, potassium thiocyanate, and ammonium thiocyanate

15. The method of claim 1, wherein the microorganism is present in laundry.

16. The method of claim 1, wherein the microorganism is present on skin, hair, mucous membranes, teeth, wounds, bruises or in the eye or oral cavity, of a human or animal.

17. The method of claim 1, wherein the composition is in the form of a soaking, washing or rinsing liquor.

18. The method of claim 1, wherein the composition is a liquid composition.

19. The method of claim 1, wherein the composition is a mouth wash, an antiinflammatory liquid, a perspirant, a deodorant, or a nasal spray.

20. The method of claim 1, wherein the composition is a solid composition.

21. The method of claim 1, wherein the composition is an eye ointment, an anti-inflammatory ointment, a foot bath salt, a perspirant, or a deodorant.

22. A method of killing or inhibiting a microorganism, comprising contacting said microorganim with a composition comprising:

(a) a peroxidase;

(b) an enhancing agent of the formula;

wherein A denotes a group —

D, —

CH═

CH—

D, —

CH═

CH—

CH═

CH—

D, —

CH═

N—

D, —

N═

N—

D, or —

N═

CH—

D;

D is selected from the group consisting of —

CO—

E, —

SO₂—

E, —

N—

XY, and —

N⁺—

XYZ;

E is —

H, —

OH,—

R, or —

OR, and X and Y and Z may be identical or different and selected from —

H and —

R;

R is C₁-C₁₆ alkyl, preferably saturated or unsaturated, branched or unbranched C₁-C₈ alkyl, optionally substituted with a carboxy, sulfo or amino group; and

B and C may be the same or different and selected from C_mH_2m+1;

1≦

m≦

5; and

(c) a hydrogen peroxide or a source of hydrogen peroxide.

24. The method according to claim 23, wherein the cosmetic product is a liquid, a gel, a paste, an ointment or a lotion.

25. The method according to claim 23, wherein the cosmetic product is a mouth wash, an eye lotion, a perspirant, a deodorant, a nasal spray, an eye ointment, or a foot bath salt.

28. The method according to claim 27, wherein the hard surface is a process equipment member of a cooling tower, a water treatment plant, a dairy, a food processing plant, a chemical process plant or pharmaceutical process plant.

29. The method according to claim 27, wherein the hard surface is a surface of water sanitation equipment.

30. The method according to claim 27, wherein the hard surface is a surface of equipment for paper pulp processing.

23. A method of preserving a cosmetic product, comprising adding to the cosmetic product an effective amount of an enzymatic antimicrobial composition comprising:

(a) a peroxidase produced by or derivable from the fungus Coprinus;

(b) an enhancing agent; and

(c) hydrogen peroxide or a source of hydrogen peroxide.

26. A method for cleaning or disinfecting contact lenses comprising contacting said contact lenses with an effective amount of an a enzymatic antimicrobial composition comprising:

(a) a peroxidase produced by or derivable from the fungus Coprinus;

(b) an enhancing agent; and

(c) hydrogen peroxide or a source of hydrogen peroxide.

27. A method of inhibiting microbial growth on a hard surface, wherein the surface is contacted with an a enzymatic antimicrobial composition comprising:

(a) a peroxidase produced by or derivable from the fungus Coprinus;

(b) an enhancing agent; and

(c) hydrogen peroxide or a source of hydrogen peroxide.