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Primer extension detection methods on active electronic microarrays

  • US 20020119484A1
  • Filed: 02/12/2002
  • Published: 08/29/2002
  • Est. Priority Date: 07/07/1994
  • Status: Abandoned Application
First Claim
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1. A method for detecting the relative amounts of at least two mRNA sequences in at least one biological sample, the method comprising:

  • (a) isolating mRNA from at least one biological sample;

    (b) amplifying at least two mRNA transcripts from each biological sample to produce amplicons, wherein the amplicons are less than about 300 bases in length, and wherein the amplification comprises a linear amplification step;

    (c) electronically hybridizing the amplicons produced in step (b) to at least two probes bound to a support at predetermined locations; and

    (d) detecting the amounts of each amplicon hybridized to the bound probes at the predetermined locations.

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