Arrays devices and methods of use thereof

• US 20020119579A1
• Filed: 09/26/2001
• Published: 08/29/2002
• Est. Priority Date: 07/14/1998
• Status: Abandoned Application

• Current Assignee: Zyomyx, Inc.
• Original Assignee: Zyomyx, Inc.
• Inventors: Wagner, Peter
• Application Number:
  US09/966,571
• Publication Number:
  US 20020119579A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00617 by chemical means
• CPC Class Codes: B01J 2219/00619 using hydrophilic or hydrophobic regions
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00641 the porous medium being continuous, e.g. porous oxide substrates
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 2319/20 containing a tag with affinity for a non-protein ligand
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: G01N 33/551 the carrier being inorganic
• CPC Class Codes: G01N 33/6845 Methods of identifying protein-protein interactions in protein mixtures

Abstract

Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells.

Claims

1. An array device for use in analyzing molecular events between one or more biomolecules and one or more analytes comprising:

a) a substrate having at least one surface;

b) one or more immobilization regions formed on said known regions of said surface(s), wherein said immobilization regions are adapted for attaching said biomolecules to said surface; and

,

c) one or more border regions formed on said surface surrounding said immobilization regions, said border region(s) having a first wettable state and a selectively achievable second wettable state different from said first wettable state.

2. The array device of claim 1 further comprising one or more convertible functional groups adapted for selectively converting between a first wettable form and a second wettable form when activated by to impart upon said border regions said second wettable state from said first wettable state.

3. The array device of claim 2, wherein said convertible functional groups are activated by an activity selected from the group consisting of photocleavage, photoisomerization, catalytic-polymerization, and photoreaction activities.

4. The array device of claim 2, wherein said convertible functional groups further comprise a first wettable state moiety attached to said surface through at least one of said convertible functional groups, wherein said first wettable state moiety imparts said first wettable state upon said border regions, and whereby removal of said first wettable state moiety from said convertible functional groups causes said border regions to attain said second wettable state.

5. The array device of claim 2, wherein said first wettable state moiety is a dendrimer or dendritic molecule.

6. The array device of claim 1, wherein said immobilization regions further comprise biomolecules immobilized within said immobilization regions.

7. A method for making an array of one or more biomolecules for use in analyzing molecular events between one or more of said biomolecules and one or more analytes comprising:

i) providing the array device comprising;

a) a substrate having at least one surface;

b) one or more immobilization regions formed on said known regions of said surface(s), wherein said immobilization regions are adapted for attaching said biomolecules to said surface; and

,

c) one or more border regions formed on said surface surrounding said immobilization regions, said border region(s) having a first wettable state and a selectively achievable second wettable state different from said first wettable state;

ii) depositing a first liquid containing at least one of said biomolecules onto at least one selected immobilization region such that said first liquid deposited is maintained within said selected region in-part by said first wettable state of at least one of said border regions;

iii) allowing at least one of said biomolecules contained in said deposited first liquid to attach to said surface within said selected immobilization region;

iv) removing said first liquid from said selected immobilization region;

v) activating said border region(s) partly or wholly maintaining said first liquid within said selected immobilization regions such that such border regions partly or wholly maintaining said liquid within said selected immobilization regions no longer are capable of maintaining said first liquid, or a second liquid within said selected immobilization region.

8. A method of carrying out a molecular reaction on a surface of an array comprising the steps of:

i) providing said array device which comprises;

a. a substrate having at least one surface;

b. one or more reaction regions formed on said known regions of said surface(s), wherein said reaction regions are adapted for reacting molecules adjacent said surface; and

,

c. one or more border regions formed on said surface surrounding said reaction regions, said border region(s) having a first wettable state and a selectively achievable second wettable state different from said first wettable state;

ii) depositing a first liquid containing at least one of said molecules onto at least one selected reaction region such that said first liquid deposited is maintained within said selected reaction region in-part by said first wettable state of at least one of said border regions;

iii) allowing at least one of said biomolecules contained in said deposited first liquid to contact said surface within said selected reaction regions;

iv) removing said first liquid from said selected reaction regions;

v) activating said border region(s) partly or wholly maintaining said first liquid within said selected reaction regions such that said border regions partly or wholly maintaining said liquid within said selected reaction regions no longer are capable of maintaining said first liquid, or a second liquid within said selected reaction regions.