Radiofrequency encoded chemical library synthesis particles method

• US 20020119580A1
• Filed: 10/05/2001
• Published: 08/29/2002
• Est. Priority Date: 04/17/1997
• Status: Abandoned Application

• Current Assignee: Central Research Fund Limited
• Original Assignee: Central Research Fund Limited
• Inventors: Corless, Anthony Robert, Wenn, David Andrew
• Application Number:
  US09/971,112
• Publication Number:
  US 20020119580A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00502 Particles of irregular geometry
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00569 EEPROM memory devices
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: C40B 70/00 Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

Abstract

A radiofrequency encoded chemical library synthesis particle which comprises a read-only radiofrequency tag linked to a solid phase. Chemical libraries synthesised on such particles and their use in biological screening methods.

Claims

1. A radiofrequency encoded chemical library synthesis particle which comprises a read-only radiofrequency tag linked to a solid phase.

2. A synthesis particle as claimed in claim 1 wherein the radiofrequency tag comprises EEPROM memory.

3. A synthesis particle as claimed in claim 1 wherein the radiofrequency tag comprises hard wired links to provide a read only memory.

4. A synthesis particle as claimed in any previous claim wherein the read-only radiofrequency tag is remotely-powered.

5. A synthesis particle as claimed in any previous claim wherein the read-only radiofrequency tag has an area of 2 mm² or smaller.

6. A synthesis particle as claimed in any one of the previous claims wherein the read-only radiofrequency tag is linked to the solid support so as to form an integral composite particle.

7. A synthesis particle as claimed in claim 6 wherein the tag is firstly encapsulated in an inert protective material.

8. A synthesis particle as claimed in claim 6 wherein the tag is associated with resin as a substantially flat faced structure.

9. A chemical library immobilised on a plurality of radiofrequency encoded synthesis particles according to any one of the previous claims, each particle having one or more members of the library on it and having a unique radiofrequency code.

10. A chemical library as claimed in claim 9 wherein each particle has only one member of the library on it.

11. A method for the preparation of a chemical library as claimed in claim 9 or claim 10 wherein the movement of each particle has been tracked during synthesis by reference to its individual tag.

12. A method as claimed in claim 11 wherein the synthesis particles are tracked using a robotic pick and place tool.

13. The use of a chemical library as claimed in claim 9 or claim 10 in screening methods to identify compounds which modulate the activity of a biological of interest.

14. The use as claimed in claim 13 wherein robotic apparatus is used to select library members on a random or directed basis for use in the screening methods.

15. The use as claimed in claim 14 wherein the directed basis is a structure/activity relationship.

16. A robotic “

pick and place”

machine adapted to read synthesis particles as claimed in any one of claims 1-8.