Chemiluminescence-based microfluidic biochip
First Claim
1. A method for performing biological assay in a self-contained microfluidic-based luminescence biochip, the method comprising the steps of:
- (a) providing said biochip with a plurality of compartments and interconnecting microchannels, said compartments having storage means for storing a plurality of samples, reagents, luminescent substrates, and reaction sites immobilized with probes; and
means for interconnecting said compartments to provide fluid transfer through said microchannels;
(b) transferring sequentially at least one of said samples and then at least one of said reagents through said microchannels to at least one of said reaction sites, said at least one sample and said at least one reagent reacting with said probes and forming a probe complex;
(c) transferring said luminescent substrate through said microchannels to said reaction sites, said luminescent substrates reacting with said probe complex and generating luminescence; and
(d) detecting said luminescence with an optical detector located above or under said reaction sites.
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Abstract
The disclosure describes how to use luminescence detection mechanism, move microfluid, and control multiple-step biochemical reactions in closed confined microfluidic biochip platform. More particularly, a self-contained disposable biochip with patterned microchannels and compartments having storage means for storing a plurality of samples, reagents, and luminescent substrates. At least one external microactuator in the biochip system produces positive pressure and automates multiple-step reactions in microfluidic platforms for clinical chemistry, cell biology, immunoassay and nucleic acid analysis. The method comprises the steps of transferring sequentially at least one of samples, reagents, and then luminescent substrate from compartments through microchannels to reaction sites. The luminescent substrates react with probes to form a probe complex resulting into luminescence, which is detected by an optical detector.
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Citations
13 Claims
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1. A method for performing biological assay in a self-contained microfluidic-based luminescence biochip, the method comprising the steps of:
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(a) providing said biochip with a plurality of compartments and interconnecting microchannels, said compartments having storage means for storing a plurality of samples, reagents, luminescent substrates, and reaction sites immobilized with probes; and
means for interconnecting said compartments to provide fluid transfer through said microchannels;
(b) transferring sequentially at least one of said samples and then at least one of said reagents through said microchannels to at least one of said reaction sites, said at least one sample and said at least one reagent reacting with said probes and forming a probe complex;
(c) transferring said luminescent substrate through said microchannels to said reaction sites, said luminescent substrates reacting with said probe complex and generating luminescence; and
(d) detecting said luminescence with an optical detector located above or under said reaction sites. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification