Method of analyzing granular composition by fluorescene analysis
First Claim
1. A method for fluorescence analysis comprising illuminating a granular composition comprising a purified biologically active compound with light capable of fluorescence excitation of a fluorescent marker comprised in the granular composition, detecting light emitted from the fluorescent marker and predicting the amount of fluorescent marker in the granular composition accessible to fluorescence excitation.
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Abstract
The present invention relates to a method for fluorescence analysis comprising illuminating a granular composition comprising a purified biologically active compound with light capable of fluorescence excitation of a fluorescent marker comprised in the granular composition, detecting light emitted from the fluorescent marker and predicting the amount of fluorescent marker in the granular composition accessible to fluorescence excitation.
21 Citations
44 Claims
- 1. A method for fluorescence analysis comprising illuminating a granular composition comprising a purified biologically active compound with light capable of fluorescence excitation of a fluorescent marker comprised in the granular composition, detecting light emitted from the fluorescent marker and predicting the amount of fluorescent marker in the granular composition accessible to fluorescence excitation.
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28. A granulation or coating apparatus comprising
(a) a granulation or coating device comprising at least one chamber for processing material into granules or coated granules, (b) an optical arrangement for performing fluorescence analysis comprising a light source for illumination of granules being processed, at least one detector capable of detecting light emitted from the granules being processed, means for projecting illuminating light onto a portion of the granules being processed, means for projecting light emitted from illuminated granules to the detector and at least one filtering device for filtering light.
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43. Use of fluorescence analysis on granules comprising a purified biologically active compound.
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44. A method according to claim comprising the steps of:
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a) providing a calibration model by illuminating a granular composition comprising a purified biologically active compound having a known quality parameter with light capable of fluorescence excitation of a fluorescent marker comprised in the granular composition, recording one or more images of the light emitted from the granular composition of a known quality and subjecting recorded images to data processing, particularly in the form of partial least squares data processing, to form a calibration model, b) illuminating a unknown granular composition comprising a purified biologically active compound with light capable of fluorescence excitation of a fluorescent marker comprised in the granular composition, recording at least one image of the light emitted from the unknown granular composition, c) comparing at least one image of the unknown granular composition with the calibration model and d) estimating the quality parameter of the unknown granular composition.
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Specification