Structure and method for performing a determination of an item of interest in a sample
First Claim
1. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
- (a) providing a sample accessible to the single structure;
(b) placing a first container for processing the sample in a first process path on the single structure;
(c) transferring the sample to the first container in the first process path;
(d) adding a reagent to the first container in the first process path;
(e) mixing contents of the first container in the first process path;
(f) separating the item of interest in the sample from the contents of the first container in the first process path;
(g) transferring the separated item of interest in the sample from the first container in the first process path to a second container in a second process path on the single structure;
(h) bringing contents of the second container to a first temperature different from a temperature of the first process path in the second process path; and
(i) detecting the item of interest in the second container in the second process path.
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Abstract
A method of performing a determination of an item of interest in a sample using a single structure is disclosed. A sample is provided accessible to the single structure. A first container for processing the sample is placed in a first process path on the single structure. The sample is transferred to the first container in the first process path. A reagent is added to the first container in the first process path. Contents of the first container is mixed in the first process path. The item of interest in the sample is separated from the contents of the first container in the first process path. The separated item of interest in the sample is transferred from the first container in the first process path to a second container in a second process path on the single structure. Contents of the second container is brought to a first temperature different from a temperature of the first process path in the second process path. The item of interest in the second container is detected in the second process path.
107 Citations
26 Claims
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1. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
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(a) providing a sample accessible to the single structure;
(b) placing a first container for processing the sample in a first process path on the single structure;
(c) transferring the sample to the first container in the first process path;
(d) adding a reagent to the first container in the first process path;
(e) mixing contents of the first container in the first process path;
(f) separating the item of interest in the sample from the contents of the first container in the first process path;
(g) transferring the separated item of interest in the sample from the first container in the first process path to a second container in a second process path on the single structure;
(h) bringing contents of the second container to a first temperature different from a temperature of the first process path in the second process path; and
(i) detecting the item of interest in the second container in the second process path. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24)
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12. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
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(a) transferring a sample to a first container in a first process path on the single structure;
(b) separating an item of interest in the sample from the contents of the first container in the first process path;
(c) transferring the separated item of interest in the sample from the first container in the first process path to a second container in a second process path on the single structure;
(d) bringing contents of the second container to a first temperature different from a temperature of the first process path in the second process path; and
(e) detecting the item of interest in the second container in the second process path.
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19. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
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(a) transferring a sample to a container in a process path on the single structure;
(b) separating an item of interest in the sample from the contents of the container in the process path;
(c) bringing contents of the container to a first temperature in the process path;
(d) bringing contents of the container to a second temperature different from the first temperature in the process path; and
(e) detecting the item of interest in the container in the process path.
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25. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
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(a) transferring a sample to a first container in a first process path on the single structure;
(b) transferring the sample from the first container in the first process path to a second container in a second process path on the single structure;
(c) bringing contents of the second container to a first temperature different from a temperature of the first process path in the second process path; and
(d) detecting the item of interest in the second container in the second process path.
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26. A method of performing a determination of an item of interest in a sample using a single structure, the method comprising the steps of:
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(a) transferring a sample to a container in a process path on the single structure;
(b) bringing contents of the container to a first temperature on the process path on the single structure;
(c) bringing contents of the container to a second temperature different from the first temperature in the process path on the single structure; and
(d) detecting the item of interest in the container in the process path on the single structure.
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Specification