Quantitative microfluidic biochip and method of use
First Claim
1. A method for performing biological assay in a microfluidic biochip platform providing constant and consistent reaction volume defining a reaction zone, the method comprising the steps of:
- (a) providing a plurality of microfluidic channels with a constant cross-section area;
(b) immobilizing at least one biological probe on said reaction zone; and
(c) transporting fluid in said microfluidic channels to said reaction zone, a portion of said fluid reacting with said at least one probe, wherein said reaction volume is product of said cross-section area multiplied with length of said microfluidic channels having said at least one biological probe.
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Abstract
Method is disclosed to provide constant and consistent amount of samples or reagent solutions for performing biological assay in a microfluidic biochip platform. A method and apparatus comprises a plurality of microfluidic channels with constant cross-section area in closed confinement, said microfluidic channels transporting fluids to at least one reaction zone immobilized with biological probes. The fluids in said microfluidic channels is transported to said reaction zone and reacted with the probes. The reaction volume, which is equal to the cross-section area multiply with the length of said channel overlaid on said reaction zone, is therefore a constant number. In addition, a method for immobilizing an array of biological probes in the microfluidic biochip arrangement, which is simple, flexible, and controllable for immobilizing, removing, and replenishing new probes is disclosed.
46 Citations
20 Claims
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1. A method for performing biological assay in a microfluidic biochip platform providing constant and consistent reaction volume defining a reaction zone, the method comprising the steps of:
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(a) providing a plurality of microfluidic channels with a constant cross-section area;
(b) immobilizing at least one biological probe on said reaction zone; and
(c) transporting fluid in said microfluidic channels to said reaction zone, a portion of said fluid reacting with said at least one probe, wherein said reaction volume is product of said cross-section area multiplied with length of said microfluidic channels having said at least one biological probe. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 13, 14, 15, 16, 17, 18, 19, 20)
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12. A method for performing biological assay in a biochip with an array of microfluidic channels providing flexible and controllable immobilization for at least one biological probe, the method comprising the steps of:
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(a) immobilizing said at least one biological probe on magnetic beads;
(b) selecting at least one of said magnetic beads and transporting said magnetic beads through one of said microfluidic channels;
(c) providing at least one external magnet beneath a portion of said microfluidic channels; and
(d) switching on said at least one external magnet for immobilization of at least one of said at least one biological probe.
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Specification