Spectroscopic system employing a plurality of data types
First Claim
1. A method of determining a condition of a test specimen, said method comprising, identifying a plurality of first test specimens having a first known condition and observing from each of said first test specimens a first fluorescence spectrum characteristic of said first known condition, obtaining an average reflectance spectrum from said plurality of first test specimens, obtaining a reflectance spectrum from a second test specimen that is observed to produce a second fluorescence spectrum that is not characteristic of said first known condition, obtaining a reflectance spectrum residual by subtracting said average reflectance spectrum from said reflectance spectrum obtained from said second test specimen, and determining said condition of said second test specimen based at least in part on an amplitude of one or more features of said reflectance spectrum residual.
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Abstract
A system and method for classifying specimens using a plurality of spectral data types. Spectra are recorded as amplitudes at a series of discrete wavelengths. Pluralities of reference spectra are recorded for specimens having known conditions. A spectrum of a first type is observed for a test specimen. The specimen is characterized as to a first known condition. In the event that the specimen does not exhibit the first known condition, a spectrum of a second type is observed and analyzed to determine which of a plurality of conditions is to be ascribed to the test specimen. In some embodiments, the test specimen can comprise human cervical tissue, and the known conditions can include normal health, metaplasia, CIN I and CIN II/III.
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Citations
23 Claims
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1. A method of determining a condition of a test specimen, said method comprising,
identifying a plurality of first test specimens having a first known condition and observing from each of said first test specimens a first fluorescence spectrum characteristic of said first known condition, obtaining an average reflectance spectrum from said plurality of first test specimens, obtaining a reflectance spectrum from a second test specimen that is observed to produce a second fluorescence spectrum that is not characteristic of said first known condition, obtaining a reflectance spectrum residual by subtracting said average reflectance spectrum from said reflectance spectrum obtained from said second test specimen, and determining said condition of said second test specimen based at least in part on an amplitude of one or more features of said reflectance spectrum residual.
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11. A spectroscopic system for determining a condition of a test specimen, comprising,
a data collection module that observes a first fluorescence spectrum characteristic of a first known condition from each of a plurality of first test specimens having said first known condition, that observes a first reflectance spectrum from each of said plurality of first test specimens, and that observes a reflectance spectrum from a second test specimen that is observed to produce a second fluorescence spectrum that is not characteristic of said first known condition, a computation module that compute an average reflectance spectrum from said first reflectance spectrum from each of said plurality of first test specimens, and that computes a reflectance spectrum residual by subtracting said average reflectance spectrum from said reflectance spectrum obtained from said plurality of second test specimens, and an analysis module that determines a condition of said second test specimen based at least in part on an amplitude of one or more features of said reflectance spectrum residual.
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21. A method of determining a disease state in a specimen, the method comprising the steps of:
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obtaining a reflectance spectrum from a test specimen having a fluorescence spectrum that is not characteristic of a healthy tissue;
subtracting an average reflectance spectrum obtained from a plurality of specimens from said reflectance spectrum to produce a reflectance spectrum residual, each producing a fluorescence spectrum characteristic of healthy tissue; and
determining disease state in said test specimen based upon one or more characteristics of said reflectance spectrum residual. - View Dependent Claims (22, 23)
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Specification