Biosensor and method of substrate quantification
First Claim
1. A biosensor comprising:
- an electrically insulating base plate;
an electrode system including a working electrode and a counter electrode formed on said base plate;
a cover member joined with said base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to said electrode system, said sample supply pathway being formed between said cover member and said base plate; and
a reagent layer formed in said sample supply pathway, wherein said reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase, an electron mediator, and a buffer having a buffering capacity in an acidic pH range.
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Abstract
Disclosed is a highly responsive biosensor comprising a cholesterol-oxidizing enzyme and cholesterol esterase. The biosensor comprises: an electrically insulating base plate; an electrode system including a working electrode and a counter electrode formed on the base plate; a cover member joined with the base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to the electrode system, the sample supply pathway being formed between the cover member and the base plate; and a reagent layer formed in the sample supply pathway, wherein the reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase, an electron mediator, and a buffer having a buffering capacity in an acidic pH range.
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Citations
20 Claims
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1. A biosensor comprising:
- an electrically insulating base plate;
an electrode system including a working electrode and a counter electrode formed on said base plate;
a cover member joined with said base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to said electrode system, said sample supply pathway being formed between said cover member and said base plate; and
a reagent layer formed in said sample supply pathway, wherein said reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase, an electron mediator, and a buffer having a buffering capacity in an acidic pH range. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 17, 20)
- an electrically insulating base plate;
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16. A measuring system including:
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(a) a biosensor comprising;
an electrically insulating base plate;
an electrode system including a working electrode and a counter electrode formed on said base plate;
a cover member joined with said base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to said electrode system, said sample supply pathway being formed between said cover member and said base plate; and
a reagent layer formed in said sample supply pathway, wherein said reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase, an electron mediator, and a buffer having a buffering capacity in an acidic pH range;
(b) voltage application means for applying a voltage between said working electrode and said counter electrode; and
(c) current detection means for detecting a current flowing between said working electrode and said counter electrode upon application of the voltage.
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18. A method of substrate quantification using a biosensor comprising:
- an electrically insulating base plate;
an electrode system including a working electrode and a counter electrode formed on said base plate;
a cover member joined with said base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to said electrode system, said sample supply pathway being formed between said cover member and said base plate; and
a reagent layer formed in said sample supply pathway, wherein said reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase and an electron mediator,said method comprising;
a pretreatment step of mixing a buffer having a buffering capacity in an acidic pH range with the sample solution;
a step of supplying the sample solution subjected to said pretreatment step to said biosensor; and
a step of determining a substrate contained in said sample solution in said biosensor.
- an electrically insulating base plate;
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19. An enzyme reagent comprising a buffer having a buffering capacity in an acidic pH range and enzymes, wherein said enzymes are at least a cholesterol-oxidizing enzyme and cholesterol esterase.
Specification