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Biosensor and method of substrate quantification

  • US 20020134676A1
  • Filed: 03/01/2002
  • Published: 09/26/2002
  • Est. Priority Date: 03/07/2001
  • Status: Active Grant
First Claim
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1. A biosensor comprising:

  • an electrically insulating base plate;

    an electrode system including a working electrode and a counter electrode formed on said base plate;

    a cover member joined with said base plate to define a sample supply pathway through which a sample solution is introduced from a sample supply port to said electrode system, said sample supply pathway being formed between said cover member and said base plate; and

    a reagent layer formed in said sample supply pathway, wherein said reagent layer contains a cholesterol-oxidizing enzyme, cholesterol esterase, an electron mediator, and a buffer having a buffering capacity in an acidic pH range.

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