Apparatus and method for electrophoresis
First Claim
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1. Apparatus for conducting electrophoresis therein, the apparatus comprising:
- a substantially closed electrophoresis chamber;
an electrophoresis gel located within said electrophoresis chamber; and
electrolyte solution in contact with said gel, wherein said electrolyte solution has high buffer capacity and low conductivity properties.
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Abstract
An apparatus and method for conducting electrophoresis, includes a cassette, gel, and electrolyte solution in contact with the gel. The electrolyte solution has high capacity and low conductivity properties, so that low volumes of electrolyte solution can be used.
75 Citations
72 Claims
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1. Apparatus for conducting electrophoresis therein, the apparatus comprising:
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a substantially closed electrophoresis chamber;
an electrophoresis gel located within said electrophoresis chamber; and
electrolyte solution in contact with said gel, wherein said electrolyte solution has high buffer capacity and low conductivity properties. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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17. Apparatus for conducting electrophoresis therein, the apparatus comprising:
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an electrophoresis chamber;
an electrophoresis gel comprising a running zone and at least one ion reservoir zone, said gel located within said electrophoresis chamber; and
electrolyte solution in contact with said electrophoresis gel, wherein said electrolyte solution has high capacity and low conductivity properties, and the volume of said at least one ion reservoir zone is less than twice the volume of said running zone of said electrophoresis gel.
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33. Apparatus for conducting electrophoresis, said apparatus comprising:
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a running gel;
an anode and a cathode at two ends of said gel; and
electrolyte solution in contact with said gel, wherein said anode is made of an electrochemically ionizable metal and wherein said electrolyte solution is of a composition such that migration of ions generated by said anode is inhibited. - View Dependent Claims (34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61)
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49. Apparatus for conducting electrophoresis, said apparatus comprising:
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a substantially closed electrophoresis chamber;
an electrophoresis gel comprising a running zone and an ion reservoir zone, said electrophoresis gel located within said electrophoresis chamber; and
an anode and a cathode at two ends of said gel; and
electrolyte solution in contact with said electrophoresis gel at least on one of said two ends, wherein said anode is made of an electrochemically ionizable metal, wherein said electrolyte solution is of a composition such that migration of ions generated by said anode is inhibited, wherein said electrolyte solution has high capacity and low conductivity properties, and wherein the volume of said ion reservoir zone is less than twice the volume of said running zone of said electrophoresis gel.
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62. A system for conducting electrophoresis, the system comprising:
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an electrical power source;
a cassette for conducting an electrophoresis therein and having conductive elements therein, said cassette comprising;
an electrophoresis gel; and
electrolyte solution in contact with said gel, wherein said electrolyte solution has high capacity and low conductivity properties; and
a support for supporting said cassette and for connecting said electrical power source to said conductive elements of said cassette. - View Dependent Claims (63, 64, 65, 66, 67, 68)
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69. A method for conducting electrophoresis in a closed cassette, comprising the steps of:
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introducing at least one test sample into a body of gel comprising a running zone;
applying an electrical field to said body of gel; and
driving an electrophoresis by providing ions for maintaining an electric field required for electrophoresis by electrolyte solution having high capacity and low conductivity properties, wherein said electrolyte solution comprises an ion reservoir zone with a volume less than twice than a volume of said running zone.
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70. A method for reducing the volume of buffer used in electrophoresis, comprising the steps of:
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providing an electrolyte solution of high buffer capacity and low conductivity;
incorporating said electrolyte solution in an electrophoresis gel at a specified pH; and
applying a voltage to said electrophoresis gel, thereby eliciting chemical reactions so as to equilibrate said specified pH.
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71. A method for inhibiting migration of an ion through an electrophoresis gel, comprising the steps of:
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providing an electrochemically ionizable anode in an electrophoresis gel;
providing an electrolyte solution within said electrophoresis gel and in contact with said anode;
applying a voltage to said electrophoresis gel so as to generate an electrochemical reaction releasing ions from said anode; and
inhibiting migration of said released ions by a chemical reaction between said released ions and components of said electrolyte solution. - View Dependent Claims (72)
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Specification
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Current AssigneeLife Technologies Limited (Thermo Fisher Scientific Incorporated)
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Original AssigneeLife Technologies Limited (Thermo Fisher Scientific Incorporated)
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InventorsUpdyke, Tim, Cabilly, Shmuel, Yogev, Uri, Margalit, Ilana
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Application NumberUS10/091,430Publication NumberTime in Patent OfficeDaysField of SearchUS Class Current204/468CPC Class CodesG01N 27/44704 Details; AccessoriesG01N 27/44747 Composition of gel or of ca...G01N 27/44782 of a plurality of samples
